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| 肥胖大鼠足细胞Nephrin、Podocin、Podoplanin的表达 | |
| 引用本文： | 林帆,罗敏,林苗.肥胖大鼠足细胞Nephrin、Podocin、Podoplanin的表达[J].福建医科大学学报,2009,43(4):283-287. |
| 作者姓名： | 林帆 罗敏 林苗 |
| 作者单位： | 1. 上海市内分泌代谢病研究所,上海,200025;福建省立医院,干部特诊一科,福州,350001 2. 上海市内分泌代谢病研究所,上海,200025 3. 福建省立医院,肾内科,福州,350001 |
| 基金项目： | 国家自然科学基金，福建省卫生厅青年科研基金 |
| 摘 要： | 目的 探讨肥胖对肾小球足细胞的损害. 方法 以高脂喂养SD大鼠16周建立肥胖动物模型,检测尿肌酐及尿微量白蛋白,透射电镜观测肾小球足细胞的形态,抽提肾皮质总RNA并逆转录,采用荧光实时定量PCR法测定足细胞相关标志物mRNA表达. 结果 与对照组相比,高脂诱导肥胖组大鼠体质量、血脂、空腹胰岛素、空腹及糖耐量后血糖、尿微量白蛋白/尿肌酐比值均明显升高,透射电镜可见肥胖组出现足突融合现象,足细胞相关标志物Nephrin、Podocin、Podoplanin mRNA的表达量在肥胖组明显升高,而SPARC、Podocalyxin、α Actinin-4、P27在两组间差别无统计学意义. 结论 高脂喂养SD大鼠16周的肥胖模型中已出现微量白蛋白尿、足突融合以及足细胞裂孔隔膜蛋白、表面阴性电荷蛋白mRNA表达量改变. |
| 关 键 词： | 肥胖症 肾小球滤过率 淋巴管生成 膜蛋白质类 疾病模型 动物 |
Changes of Podocyte-Associated Molecules Nephrin,Podocin and Podoplanin in Obese Rat |
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| LIN Fan,LUO Min,LIN Miao.Changes of Podocyte-Associated Molecules Nephrin,Podocin and Podoplanin in Obese Rat[J].Journal of Fujian Medical University,2009,43(4):283-287. | |
| Authors: | LIN Fan LUO Min LIN Miao |
| Institution: | LIN Fan1,2,LUO Min1,LIN Miao31.Shanghai Institute of Endocrine and Metabolic Diseases,Shanghai 200025,China2.Department of Exclusive Medical Care,Fujian Provincial Hospital,Fuzhou 350001,China3.Department Division of Nephrology,China |
| Abstract: | Objective To examine the obesity-related changes of podocyte-associated molecules nephrin,podocin and podoplanin in the kidney glomerulus of obese rats. Methods The urinary albumin and creatinine of 12 male obese SD rats fed with high fat food were monitored.The ultrastructural changes of podocyte of kidney glomerulus were investigated under electron microscopy,and mRNA expression profile of several podocyte-associated molecules was examined by real-time PCR. Results High fat food administration results in ... |
| Keywords: | obesity glomerular filtration rate lymphangiogenesis membrane proteins disease models animal |
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