涎腺肿瘤端粒酶的检测及其临床意义

目的 :研究涎腺肿瘤中的端粒酶活性表达 ,探讨其作为涎腺肿瘤标志物的可行性及其临床意义。方法 :用银染 TRAP法检测 2 8例涎腺癌组织及其相应的 2 8例癌旁组织、10例腮腺混合瘤、6例腮腺腺淋巴瘤、5例正常涎腺组织的端粒酶活性。结果 :2 8例涎腺癌组织中 ,有 2 5例端粒酶活性表达阳性 (89.3% )。 2 8例癌旁组织中 ,有 4例端粒酶表达阳性 (14 .3% )。 10例腮腺混合瘤 ,6例腮腺腺淋巴瘤 ,5例正常涎腺组织端粒酶表达均为阴性。涎腺癌组织端粒酶活性表达与该肿瘤的临床病理特征无明显差异 (P >0 .0 5 )。结论 :端粒酶活性表达可作为诊断涎腺癌的肿瘤标志物 ;其癌旁组织端粒酶活性检测可作为肿瘤手术治疗后监测的指标之一。

Detection of telomerase activity in salivary tumor and its significance

Objective To investigate telomerase activity in human salivary cancer and corresponding adjacent tissues and to explore the possibility of telomerase as a tumor marker and its clinical significance. Methods Twenty eight salivary cancers, 28 adjacent peritumoral tissues, 10 mixed tumors, 6 adenolymphomas, and 5 normal salivary tissues were examined for telomerase activity by the silver staining TRAP assay based on PCR. Results Twenty five of the 28 salivary cancers and 2 of the 28 adjacent peritumoral tissue specimens were positive for telomerase activity with a positive rate of 89.3% and 6.3%,respectively. Telomerase activity was negative in the 10 mixed tumors,6 adenolymphomas, and 5 normal salivary tissues. There was no correlation between the clinical stage of salivary cancer and its expression of telomerase activity (P>0.05). Conclusion Positive telomerase activity occurs in the majority of salivary cancers examined.It can be used as a tumor marker in the diagnosis of salivary cancer. Detection of telomerase activity in the adjacent peritumoral tissues can be used as a monitoring marker after treatment.