肺鳞癌组织中淋巴管分布和存在状态分析

目的探讨肺鳞癌组织中淋巴管分布和存在状态及可能的原因。方法肺鳞癌组40例，肺炎性假瘤组10例。用LYVE-1／CD34免疫组化双染色法区分淋巴管和血管并观察淋巴管内皮透明质酸受体-1(LYVE-1)蛋白表达的情况。结果1．肺鳞癌组织周边部淋巴管密度远大于内部和炎性假瘤组织。2．网状淋巴管肺鳞癌组织周边部远大于肺鳞癌组织内部及炎性假瘤组织。塌陷淋巴管肺鳞癌组织周边部密度小于肺鳞癌组织内部而大于炎性假瘤组织，塌陷淋巴管肺鳞癌组织内部密度远大于炎性假瘤组织；3．在肺鳞癌组织周边部：单腔淋巴管密度远大于塌陷淋巴管密度和网状淋巴管密度，塌陷淋巴管密度小于网状淋巴管密度。在肺鳞癌组织内部：单腔淋巴管密度小于塌陷淋巴管密度而大于网状淋巴管密度，塌陷淋巴管密度大于网状淋巴管密度。在炎性假瘤组织：单腔淋巴管密度远大于塌陷淋巴管密度和网状淋巴管密度。结论肺鳞癌组织淋巴管生成主要在周边部，肺鳞癌组织内部淋巴管塌陷明显增多导致的淋巴管无功能状态可能主要是由于高间质压引起的。

Investigation of distribution and existing status in pulmonary squamous cell carcinoma tissue

Objective: To investigate the distribution and existing statues in pulmonary squamous cell carcinoma tissue and its possible causes. Methods: Double immunohistochemical staining for LYVE-1/CD34 for 40 cases of pulmonary squamocellular carcinoma and 10 cases of pulmonary inflammatory pseudotumor were performed to distinguish lymphoducts and blood vessels, and to observe the lymphatic vessel endothelial HA receptor-1(LYVE-1) manifestation. Results: 1. The density of lymphatic vessels was elevated in the perimeter of the pulmonary squamocellular carcinoma tissue relative to the interior of that and the pulmonary inflammatory pseudotumor normal tissue. 2. The reticular lymphoducts in the perimeter of the pulmonary squamocellular carcinoma tissue was higher than that in the interior and the pulmonary inflammatory pseudotumor. The density of collapsed lymphoducts in the perimeter of the pulmonary squamocellular carcinoma tissue was lower than that in the interior and higher than that in the pulmonary inflammatory pseudotumor. The density of collapsed lymphoducts in the interior of the pulmonary squamocellular carcinoma tissue was much higher than that in the pulmonary inflammatory pseudotumor. 3. The density of single lumen lymphoducts was much higher than that of collapsed and reticular lymphoducts and the density of collapsed lymphoducts was lower than that of reticular lymphoducts in the perimeter of the pulmonary squamocellular carcinoma tissue. The density of single lumen lymphoducts was lower than that of reticular lymphoducts and higher than that of reticular lymphoducts in the interior of the pulmonary squamocellular carcinoma tissue. The density of collapsed lymphoducts was higher than that of reticular lymphoducts in the interior of the pulmonary squamocellular carcinoma tissue. The density of single lumen lymphoducts was much higher than that of collapsed and reticular lymphoducts in the pulmonary inflammatory pseudotumor. Conclusions: The production of lymphoducts is mainly in the perimeter of the pulmonary squamocellular carcinoma tissue. The clearly accelerated number of collapsed lymphoducts in the interior of the pulmonary squamocellular carcinoma tissue which lead to the dysfunction of lymphoducts mainly due to the high interstitial pressure.