| 百日咳杆菌效应CpG-ODNs调控变应性哮喘Toll信号分子的实验研究 |
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| 作者姓名： | Zhang BY Chi S Sun Y |
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| 作者单位： | 扬州大学医学院药理学教研室; |
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| 摘 要： | 从百日咳杆菌中分离出非甲基化寡核苷酸单链(CpG-ODNs),建立卵白蛋白(OVA)致敏的小鼠急性哮喘与慢性哮喘模型,经急性哮喘模型从百日咳杆菌CpG-ODNs与9条相似度高且含有CG片段的seq A～seq I序列中筛选出有效序列(seq A),以seq A腹腔注射(ip)及灌胃(ig)对急、慢性哮喘小鼠分别进行干预。检测支气管肺泡灌洗液(BALF)中白细胞(WBC)总数和嗜酸性粒细胞(EOS)数,酶联免疫吸附法(ELISA)检测BALF中细胞因子及血管内皮生长因子(VEGF)水平。逆转录聚合酶链式反应(RT-PCR)检测小鼠脾细胞TLR-9 mRNA表达量;蛋白印迹(Western blotting)法检测小鼠肺组织TLR-9蛋白表达。结果表明,小鼠急性哮喘模型经腹腔注射及灌胃seq A预处理,可明显抑制BALF中WBC总数增加,显著抑制EOS增加;百日咳杆菌、CpG+及seq A～seq D灌胃组亦显著抑制EOS增加,而seq E～seq I作用不明显;上述各序列干预组以腹腔注射途径抑制EOS作用较为显著。对小鼠慢性哮喘模型,CpG+和seq A腹腔注射预处理均能明显上调BALF中γ干扰素(IFN-γ)...
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| 关 键 词： | 哮喘 百日咳杆菌 寡核苷酸单链 Th1/Th2 TLR-9 |
Modulation of Toll-like signal path of allergic asthma by CpG-ODNs from Bordetella pertussis |
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| Zhang BY,Chi S,Sun Y.Modulation of Toll-like signal path of allergic asthma by CpG-ODNs from Bordetella pertussis[J].Acta Pharmaceutica Sinica,2011,46(3):285-292. |
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| Authors: | Zhang Bao-Yuan Chi Shen Sun Yun |
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| Institution: | ZHANG Bao-yuan,CHI Shen,SUN Yun(Department of Pharmacology,Medical College of Yangzhou University,Yangzhou 225001,China) |
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| Abstract: | This study focused on prevention and treatment of acute and chronic asthma by oligonucleotides containing unmethylated CpG motifs(CpG-ODNs).Acute and chronic asthma models of mice were made by sensitizing and inhaling ovalbumin(OVA);The number of white blood cells(WBC) and eosnophils(EOS) in bronchoalveolar lavage fluid(BALF) was counted and the concentration of cytokines and vascular endothelial growth factor(VEGF) was examined in BALF by ELISA kit.After that,TLR-9 mRNA was detected in mice spleen cells by... |
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| Keywords: | asthma Bordetella pertussis oligodeoxynucleotides Th1/Th2 Toll like receptor-9 |
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