| 羟基红花黄色素A缓解脂多糖诱导家兔白细胞活化的作用 |
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| 作者姓名： | Wu W Jin M Tong J Wang XF Zang BX |
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| 作者单位： | 首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所; |
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| 基金项目： | 国家自然科学基金资助项目(30572344) |
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| 摘 要： | 红花是一种常用的活血化瘀药,羟基红花黄色素A(HSYA)是其主要的活性成分。为了研究HSYA对脂多糖(LPS)诱导的白细胞(PMN)损伤的保护作用,采用比色法来观察HSYA对LPS诱导PMN的黏附作用、Fura-2法测定PMN内Ca2+浓度、RT-PCR法测定TNF-α、IL-6 mRNA表达水平及免疫荧光染色法观察NF-κB核移位的情况。实验结果显示:HSYA浓度为1.01×10-4 mol·L-1时就可明显缓解LPS诱导的家兔PMN黏附活性增强;浓度为3.1×10-5 mol·L-1时可抑制细胞内游离Ca2+浓度升高;浓度为5.2×10-5 mol·L-1时可缓解TNF-α、IL-6 mRNA表达水平的升高,并可抑制NF-κB p65亚基的核移位。结果说明,HSYA对LPS诱导的PMN活化具有缓解作用。
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| 关 键 词： | 羟基红花黄色素A 脂多糖 白细胞 |
Inhibitory effect of hydroxysafflor yellow A against PMN activation induced by LPS |
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| Wu W,Jin M,Tong J,Wang XF,Zang BX.Inhibitory effect of hydroxysafflor yellow A against PMN activation induced by LPS[J].Acta Pharmaceutica Sinica,2011,46(2):153-157. |
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| Authors: | Wu Wei Jin Ming Tong Jing Wang Xiao-fei Zang Bao-xia |
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| Institution: | activation induced by LPS WU Wei,JIN Ming,TONG Jing,WANG Xiao-fei,ZANG Bao-xia(Beijing Anzhen Hospital,Capital Medical University-Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029,China) |
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| Abstract: | Carthamus tinctorius L.is a traditional Chinese medicine with the effect of promoting blood circulation and removing blood stasis.HSYA(hydroxysafflor yellow A) is the main effective component of Carthamus tinctorius L.In order to study the inhibitory effects of HSYA against PMN(polymorphonuclear) activation induced by LPS(lipopolysaccharide),rabbit PMN adhesion potency which was activated by LPS through colorimetry method was observed.Cellular free calcium concentration was determined by fluorescence spectr... |
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| Keywords: | hydroxysafflor yellow A lipopolysaccharide polymorphonuclear |
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