三氧化二砷对低氧下人胃癌细胞SGC-7901生长活性及凋亡的影响

目的探讨三氧化二砷（As2O3）在低氧条件下对体外培养的人胃癌细胞SGC-7901生长的抑制及凋亡影响。方法用氯化钴（CoC l2）制作低氧模型,设常氧组、低氧组、低氧加药物组,分别取0.5、1.0、2.0、5.0、10.0μmol/L五个不同浓度的As2O3作用于胃癌细胞,用细胞活力测定（MTT）法检测药物作用后的细胞活力,HOECHST33258染色试剂盒测细胞凋亡。结果①低氧加药物组As2O3对SGC-7901细胞生长有抑制作用,与常氧对照组比较差异有统计学意义（P〈0.05）,并呈剂量-时间-效应关系。②低氧加药物组中,随着As2O3浓度的升高,胃癌细胞凋亡率也逐渐升高（P〈0.05）。结论低氧下As2O3对人胃癌细胞SGC-7901生长有抑制和诱导凋亡作用,这种作用可能是As2O3发挥抗肿瘤的生物学基础。

Influence of arsenic trioxide on the growth activity and apoptosis of gastric cancer cell during hypoxia

Objective To investigate the effect of arsenic trioxide (As2O3) on the inhibition and apoptosis of gastric cancer cell SGC-7901 during hypoxia. Methods The environment of hypoxia was established by CoCl2 method. The gastric cancer cell were divided into three groups:normal group, hypoxia group and hypoxia combined arsenic trioxide group. The gastric cancer cell SGC-7901was stimulated respectivly 0.5,1.0,2.0,5.0 μmol/L and 10.0 μmol/L of arsenic trioxide.The growth activity of the gastric caner cell of As2O3 was determined by MTT ,and the apoptosis of the gastric caner cell by arsenict rioxide were examined with HOECHST33258 of gastric cancer cell SGC-7901, there was a significant difference rate gradually increased with higher concentration of As2O3 in hypoxia combined arsenic trioxide groups(P＜0.05). Conclusion The growth of human gastric cancer cell SGC-7901 were suppressed, and apoptosis of human gastric cancer cell SGC-7901were induced by As2O3 during hypoxia,it may be biological basic of anti-tumor of As2O3.