载阿霉素聚氰基丙烯酸正丁酯纳米粒对L-02细胞的毒性研究

目的:检验载阿霉素聚氰基丙烯酸正丁酯纳米粒(ADM-PBCA-NP)对人肝细胞株L-02细胞的毒性。方法:体外培养人肝细胞株L-02，检测各组不同纳米粒浓度培养上清液中乳酸脱氢酶（LDH）的活性；用噻唑蓝（MTT）法检测L-02在与ADM-PBCA-NP，阿霉素（ADM）和空白纳米粒(PBCA-NP)共同培养的条件下对肝细胞的毒性，通过对不同浓度PBCA-NP的溶血试验测定其生物相容性。结果:MTT结果显示，ADM组，ADM-NP组，PBCA-NP组在 10-6 mol/L的浓度范围内，细胞毒性均为1级，即对细胞无害。各组肝细胞L-02培养上清液中LDH活性的测定无统计学差异。结论:在10-6 mol/L浓度范围内，ADM-NP和PBCA-NP对肝细胞L-02无明显毒性作用；在一定的浓度范围内细胞相容性良好，不会引起溶血。

A study on cytotoxicity of ADM-PBCA-NP to L-02 cells

Abstract:Objective:To investigate the cytotoxicity of ADM-PBCA-NP on L-02 cells.Methods : L-02 cells were cultured in vitro and the LDH activity of supernatant liquid of culture cells was examined. The toxicity of ADM-PBCA-NP, ADM and PBCA-NP to L-02 cells by the MTT assay was also determined, and the haemolysis function of PBCA-NP with different concentrations was detected.Results:The cytotoxicity of ADM-PBCA-NP, ADM and blank PBCA nanoparticles to L-02 cells under the 10-6 mol/L concentration range was not cytototic (grade 1). LDH activity of supernatant liquid of culture cells showed no differences between the 3 groups.Conclusions:Nanoparticles of ADM-NP and PBCA-NP in the 10-6 mol/L concentration range have no significant toxic effect on L-02 hepatic cells; and in a certain concentration range, the cell compatibility is excellent and will not lead to hemolysis.