| 人免疫缺陷病毒基因的亚克隆 |
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| 引用本文： | 吉昌华 杨立宏. 人免疫缺陷病毒基因的亚克隆[J]. 医学争鸣, 1992, 13(4): 264-267 |
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| 作者姓名： | 吉昌华 杨立宏 |
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| 作者单位： | 第四军医大学生物化学教研室(吉昌华，杨立宏，毛积芳)，第四军医大学生物化学教研室(苏成芝) |
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| 摘 要： |
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| 关 键 词： | 免疫缺陷病毒 基因 亚克隆 质粒 |
Subcloning of human immunodeficiency virus type I genes |
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| JI Chang-Hua,YANG Li-Hong,MAO Ji-Fang and SU Cheng-Zhi. Subcloning of human immunodeficiency virus type I genes[J]. Negative, 1992, 13(4): 264-267 |
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| Authors: | JI Chang-Hua YANG Li-Hong MAO Ji-Fang SU Cheng-Zhi |
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| Affiliation: | JI Chang-Hua,YANG Li-Hong,MAO Ji-Fang and SU Cheng-Zhi Department of Biochemistry |
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| Abstract: | We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. Digested with Kpn I, the recombinant plasmid pARV-2/7A was cleaved into 4 fragments. The 6.0 kb fragment was recircularized, and the other three fragments were ligated with pUC19 plasmid cut open by Kpn I. By screening and identification, four subclones were obtained. Of which, pJE332 contains the whole env gene, pJG423 contains LTR, gag and part of pol gene, pJP163 and pJP032 contain only part of pol gene. These subclone plasmids have been being used in many investigations on gene detection and cloning of HIV type I. |
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| Keywords: | human immunodeficiency virus AIDS associated retrovirus gene subcloning restriction enzyme plasmid |
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