口腔粘膜下纤维化、白斑及鳞癌上皮细胞内皮素1表达的定量分析

目的 :探讨内皮素 (ET 1)在口腔癌变发病机制中的作用和意义。方法 :采用免疫组化染色SABC法和图象分析技术 ,对人口腔粘膜下纤维化 (OSF) 10例、白斑 (OLK) 9例、鳞状细胞癌 (SCC) 14例及正常口腔粘膜(NOR) 10例的上皮细胞ET 1表达进行定量分析。结果 :①ET 1在OSF、OLK、SCC组织中的表达增强 ,阳性颗粒主要位于上皮棘细胞、基底细胞的胞浆胞膜上。ET 1表达阳性率和含量显著高于正常对照 (P <0 .0 1)。②OLK、SCC上皮细胞ET 1含量呈显著增加趋势 (P <0 .0 5 )。③OSF上皮细胞ET 1含量显著高于OLK(P <0 .0 5 ) ,与SCC相比无显著性差异 (P >0 .0 5 )。结论 :ET 1含量在口腔癌前病变至癌变过程中可能存在一种量变关系 ,OSF中ET 1过量表达可能提示其上皮细胞的癌变潜能 ,ET 1与OSF、OLK、SCC的发生发展密切相关

Immunohistochemical and quantitative observation of endothelin-1 expression in biopsies from oral submucous fibrosis, oral leukoplakia and oral squamous cell carcinoma

Objective: To investigate the effects of endothelin -1(ET-1) in the process of oral carcinogenesis. Methods: A sensitive SABC immuno-histochemical method and image pattern analysis technique were adopted in the examination of the localization and content of ET-1 in human oral mucosa from 10 cases of oral submucous fibrosis (OSF), 9 of oral leukoplakia (OLK),14 of oral squamous cell carcinoma(SCC)and 10 of normal control(NOR). Results: (1)Increaed expression of ET-1 was observed in the epithelial cells in OSF, OLK and SCC.The immune reative material was prominent within the cytoplasm and on the cell membrane of spinocells and basiocells.The proportion of ET-1 positive cells and cellular content of ET-1 in OSF,OLK and SCC were all significantly higher than that in NOR (P<0.01).(2)The cellular content of ET-1 increased remarkably in OLK and SCC (P<0.05). (3) The cellular content of ET-1 in OSF was significantly higher than that in OLK(P<0.05),but no significant difference was found between OSF and SCC(P>0.05). Conclusion: The cellular content of ET-1 probably increases in the process from premalignance to malignance.Its overexpression in OSF possibly indicates the potential malignance of epithelial cells.ET-1 may play a possible role in the pathogenesis of OSF,OLK and SCC.