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Poloxamer 188 failed to prevent exercise-induced membrane breakdown in mdx skeletal muscle fibers |
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| Authors: | Quinlan John G Wong Brenda L Niemeier Richard T McCullough Alexandra S Levin Linda Emanuele Martin |
| Affiliation: | aDepartment of Neurology, The University of Cincinnati, 4010 Medical Science Building, 231 Albert Sabin Way, Cincinnati, OH 45267-0525, USA bDepartments of Epidemiology and Statistics, The University of Cincinnati, Cincinnati, OH, USA cCincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA dSynthRx Corporation, 150 Technology Parkway, Norcross, GA, USA |
| Abstract: | We sought to determine the effectiveness of poloxamer 188 (P188) in protecting dystrophin-deficient, mdx skeletal muscle fiber membrane against exercise-induced breaches. mdx mice were treated with either P188 or placebo via intraperitoneal injections and run on a treadmill for 60–90 min. Membrane breakdown was quantified in cross-sections of rectus femoris muscle pretreated with Evans blue dye (in vivo). The mean % dye-penetrated muscle in the P188 and placebo groups was not significantly different in each of three trials. These results contrast with a recent report of P188 being highly effective in protecting the stretch- and dobutamine-stressed mdx heart muscle. The most likely explanations for the disparity are: (1) the exercise stress we used was beyond the protective range of P188, (2) P188 delivery and serum concentration were sub-optimal, or (3) the mdx skeletal myopathy and cardiomyopathy have fundamentally different responses to treatment. |
| Keywords: | mdx mouse Poloxamer 188 Sarcolemma Dystrophin-deficient Exercise |
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