| 人类杀伤细胞免疫球蛋白样受体基因PCR-SSP分型方法的建立及其应用 |
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| 引用本文： | 姜侃,朱发明,章伟,吕沁风,何吉,傅启华,严力行. 人类杀伤细胞免疫球蛋白样受体基因PCR-SSP分型方法的建立及其应用[J]. 中华微生物学和免疫学杂志, 2005, 25(12): 1044-1048 |
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| 作者姓名： | 姜侃 朱发明 章伟 吕沁风 何吉 傅启华 严力行 |
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| 作者单位： | 1. 310003,杭州,浙江大学医学院附属第一医院;310006,杭州,浙江省血液中心,输血研究所
2. 310006,杭州,浙江省血液中心,输血研究所 |
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| 基金项目： | 浙江省自然科学基金项目（Y204216）;浙江省医药卫生科学研究基金项目（2004A014） |
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| 摘 要： | 目的建立PCR-SSP方法检测人类杀伤细胞免疫球蛋白样受体（KIR）基因。方法参照K／R基因数据库，采用计算机软件设计一系列特异性引物建立PCR-SSP方法，以人类生长激素基因特异性引物作为内对照，以Dynal公司的KIR PCR-SSP分型试剂盒作为验证。结果本实验所设计的KIR特异性引物可检测出所有K／R基因，扩增产物条带清晰，特异性强。结论本实验建立的KIR PCR-SSP方法结果准确，具有可行性。
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| 关 键 词： | PCR-SSP 杀伤细胞免疫球蛋白样受体 基因型 |
| 收稿时间： | 2005-05-30 |
| 修稿时间： | 2005-05-30 |
The establishment of PCR-SSP method for killer cell immunoglobulin-like receptor gene family and its application |
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| JIANG Kan,ZHU Fa-ming,ZHANG Wei,L Qin-feng,HE Ji,FU Qi-hua,YAN Li-xing. The establishment of PCR-SSP method for killer cell immunoglobulin-like receptor gene family and its application[J]. Chinese Journal of Microbiology and Immunology, 2005, 25(12): 1044-1048 |
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| Authors: | JIANG Kan ZHU Fa-ming ZHANG Wei L Qin-feng HE Ji FU Qi-hua YAN Li-xing |
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| Affiliation: | The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China |
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| Abstract: | Objective To establish a PCR-SSP method to detect all KIR genes. Methods Nineteen pairs of primers were designed to detect the different KIR genes, with human growth hormone specific primer added as the control, and KIR PCR-SSP kit was used for confirmation. Results The PCR-SSP method could detect all KIR genes, and the PCR products show high specificity. Conclusion The PCR-SSP method is reliable and is recommended. |
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| Keywords: | PCR-SSP KIR Genotype |
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