| 槽式对流免疫电泳法检测可提取性核抗原抗体的研究 |
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| 引用本文： | 刘学明,郑德明,魏景艳,张晓慧,孙荣武. 槽式对流免疫电泳法检测可提取性核抗原抗体的研究[J]. 吉林大学学报(医学版), 1995, 0(2) |
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| 作者姓名： | 刘学明 郑德明 魏景艳 张晓慧 孙荣武 |
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| 作者单位： | 长春中医学院附属医院免疫研究室，吉林省医院检验科 |
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| 摘 要： | 用引进的槽式对流免疫电泳法（槽ＣＩＥ）和国内常规的免疫双扩散法（ＩＤ），同时对８９例病人血清可提取性核抗原（ＥＮＡ）的抗体进行了测定和比较。槽ＣＩＥ法图像清晰、沉淀线呈弧型，结果明显比ＩＤ法容易判断，ＥＮＡ抗体阳性病人血清的检出率非常显著地高于ＩＤ法（Ｐ＜０．００１），抗ＳＭ、ＲＮＰ、ＳＳＡ、ＳＳＢ等每种抗休的检出率也均高于ＩＤ法，其中前３种差异非常显著（分别Ｐ＜０．０１，Ｐ＜０．０１，Ｐ＜０．００１）。
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| 关 键 词： | 对流免疫电泳，ENA抗体 |
Investigations on CIE method in the detection of anti ENA antibodies |
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| Liu Xueming,Zheng Deming,Wei Jengyan,et al. Investigations on CIE method in the detection of anti ENA antibodies[J]. Journal of Jilin University: Med Ed, 1995, 0(2) |
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| Authors: | Liu Xueming Zheng Deming Wei Jengyan et al |
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| Abstract: | For the detection of anti ENA antibodies an improved method of counterimmu-noeletrophoresis(Trough-CIF)was introduced and compared with the routine test of double im-munodiffusion( ID).Eighty nine patients sera were tested by these two methods.The results were shown as follows.(1) Picturcs and results in Trough-CIE were clearer and easier to be judged thanin ID(2).Detectable rate of anti ENA positive sera was significantly higher in Trough-CIE than inID tP<0.001).(3)Each of four kinds of ENA antibodies(anti-SM,anti-RNP,anti-SSA andanti-SSB)was more frequently detected by Trough-CIE than by ID,especially the first three kindsof antibod000(P<0.01,P<0.01,P<0.001 respectively) |
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| Keywords: | counterimmunoelectrophoresis(CIE) ENA antibodies。 |
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