血液流变学中全血表观黏度与Casson黏度及屈服应力的分析和意义

背景全血黏度是血液流变学研究的重点,对于揭示因血液流变性改变而出现的心肌梗死、脑梗死等缺血性疾病的发生具有重要意义.目的研究血液的宏观与微观流变学特征,探寻血液流变学测量方法的标准化.设计以健康体检者的静脉血为纳入对象的对比实验.单位桂林医学院物理教研室.材料随机选取86例健康体检者的静脉血于桂林医学院血液流变学研究室完成实验.方法本实验86例全血样品,在7个剪变率(0.6,2,5,10,20,40,100s-1)下,顺序从低剪变率到高剪变率,再从高剪变率到低剪变率进行检测,对两个过程的测量值进行比较.主要观察指标①主要结局两个测量过程7个剪变率下的表观黏度值.②次要结局两个测量过程下的Casson黏度、屈服应力.结果两个测量过程,同一剪变率下的表观黏度值,除0.6s-1对应的表观黏度值有显著性差异外(P＜0.05),其余各表观黏度值无显差异(P＞0.5),Casson黏度和屈服应力值无显著性差异(P＞0.5,P＞0.3).结论实验研究中测量Casson黏度、屈服应力值有利于优化血液流变学检测指标和测量方法的标准化,且对同一血样重复测试表观黏度、Casson黏度和屈服应力值是可行的.对于揭示血液流变性的改变,以及心肌梗死、脑梗死、高血压、冠心病等缺血性疾病的发生和发展机理具有重要意义.

Analysis and significance of whole blood apparent viscosity,Casson viscosity and yield stress in hemorheology

BACKGROUND: Whole blood viscosity is the keystone in hemorheological research, which has important significance in the exposure of the generation of ischemic diseases including myocardial infarction (MI) and cerebral infarction(CI) due to hemorheological changes.OBJECTIVE: To investigate the characters of macroscopical and microscopical hemorheology to explore the standardization of hemorheological measuring method.DESIGN: An observatory comparative study based on the venous blood of healthy individual.SETTING: Department of physics in a medical college.MATERIALS: Venous bloods of 86 healthy individuals were randomly selected for the study, which was conducted in the Laboratory of Hemorheology of Guilin Medical College.METHODS: Totally 86 whole blood samples were tested under 7 shear rates (0.6 s-1,2 s-1,5 s-1, 10 s-1, 20 s-1, 40 s-1, 100 s-1) from low shear rate to high shear rate, and then from high shear rate to low shear rate. The measuring value of the two processes was compared.Casson viscosity and yield stress in two measuring process.RESULTS: There was no significant difference of apparent viscosity( P＞ 0.05 ) except the 0.6 s- 1 -corresponding apparent viscosity ( P ＜ 0.05 )at some shear rate in two measuring process. There was no significant difference in Casson viscosity and yield stress( P ＞ 0.5, P ＞ 0. 3).CONCLUSION: The measurement of Casson viscosity and yield stress is good for the optimization of the standardization of hemorheological indicators and measuring method; and it is feasible to test apparent viscosity, Casson viscosity and yield stress repeatedly in same blood sample, which has important significance in the exposure of the hemorheological changes and the generation and development mechanism of ischemic diseases including MI,CI, hypertension, and coronary heart disease(CHD).