血清APN、PAI-1与糖尿病视网膜病变的相关性研究

目的探讨脂联素（APN）和纤溶酶原激活物抑制物-1（PAI-1）在糖尿病视网膜病变（DR）时的变化规律及临床意义。方法 ELISA法检测2型糖尿病无DR患者30例（NDR组）、2型糖尿病合并DR背景期患者30例（BDR组）、2型糖尿病合并DR增殖期患者30例（PDR组）和健康对照30例（NC组）的血清APN和PAI-1水平,氧化酶法测定FPG、TC、TG、HDL-C、LDL-C水平,免疫抑制比浊法测定HbA1c水平,计算BMI。结果 NC组、NDR组、BDR组、PDR组血清APN和PAI-1水平组间比较差异均有统计学意义（P〈0.05）。血清APN与BMI、FPG、LDL-C、TG呈负相关,与HDL-C呈正相关;PAI-1与TG、HbA1c、BMI呈正相关。结论 APN和PAI-1可能参与了DR的发生和发展,高TG血症、肥胖、高血糖可能是导致PAI-1升高和APN降低的原因。

Study of relationship between adiponectin, plasminogen activator inhibitor-1 and diabetic retinopathy

Objective To explore the regularity and clinical significance of adiponectin and plasminogen activator inhibitor-1（PAI-1） in diabetic retinopathy（DR）.Methods Adiponectin and PAI-1 levels were detected by using ELISA method in NDR group（30 non-DR patients with type 2 diabetes mellitus）,BDR group（30 DR patients with type 2 diabetes mellitus）,PDR group（30 proliferative DR patients with type 2 diabetes mellitus） and NC group（30 healthy controls）.The levels of FPG,TC,TG,HDL-C,LDL-C and HbA1c were measured by glucose oxidase method or immunosuppression turbidmetry method,and the BMI was calculated.Results Levels of adiponectin and PAI-1 had significant differences among the four groups（P〈0.05）.Serum adiponectin was negatively correlated with BMI,FPG,LDL-C,TG,and positively correlated with HDL-C.PAI-1 was positively correlated with TG,HbA1c and BMI.Conclusion Adiponectin and PAI-1 may be involved in the occurrence and development of DR,which were affected by TG,obesity and hyperglycaemia.