芒果叶醇提取物对小鼠急性酒精性肝损伤的影响

目的: 研究芒果叶醇提取物(MLE)对小鼠急性酒精肝损伤的影响。 方法: 18~22 g 昆明种小鼠,雌雄各半,随机分为正常组、模型组、阳性药(护肝片1.5 g·kg^-1)组,MLE高、中、低(15.0,5.0,2.5 g·kg^-1)剂量组,每组10只。小鼠1次性灌胃50%乙醇(12 mL·kg^-1)建立急性酒精性肝损伤模型,测定血清中丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)活性及甘油三酯(TG) 含量,检测肝组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)与丙二醛(MDA),并进行肝组织病理切片检查。 结果: 与模型组比较,MLE高、中、低剂量组血清TG与肝组织MDA含量及肝损伤病理积分均明显降低(P<0.05);MLE高、中剂量组小鼠血清ALT与AST活性明显降低(P<0.05),肝组织GSH水平明显升高(P<0.05);MLE高剂量组肝组织T-SOD活性明显升高(P<0.05)。 结论: MLE对小鼠急性酒精肝损伤具有保护作用,可能与其抗脂质过氧化作用相关。

Effect of Mango Leaf Extract on Acute Alcoholic Liver Injury in Mice

Objective: To observe the effect of mango leaf extract (MLE) on acute alcoholic liver injury in mice. Method: Sixty mice were randomly divided into control group, model group, positive drug group(Hugan tablet 1.5 g·kg^-1), MLE high, middle and low-dose(15.0, 5.0, 2.5 g·kg^-1)group, 10 each group. Acute liver injury model was induced by 50% alcohol given by gavage. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), triglycerides(TG) in the serum and malondialdehyde(MDA), glutathione(GSH), total superoxide dismutase (T-SOD) in the liver tissue were measured. Liver index was calculated and pathological changes of the liver tissue were observed under optical microscope. Result: Compared with model group,the serum TG in MLE high, middle and low dose group significantly lowered (P<0.05);the serum ALT, AST in MLE high, middle dose group significantly lowered (P<0.05);T-SOD activity in MLE high dose group rose obviously (P<0.05);GSH levels of liver tissue in MLE high and middle dose group rose obviously (P<0.05);MDA content in liver tissue in MLE high, middle and low dose group lowered obviously (P<0.05);the liver pathological score of MLE high, middle and low dose group lowered significantly (P<0.05). Conclusion: MLE has a protective effect on acute liver injury induced by alcohol in mice,which may be related to its anti-lipid peroxidation.