5种中药乙醇提取物的体外抗氧化活性研究

目的: 研究马尾松等5种中药的乙醇提取物的体外抗氧化活性。 方法: 采用乙醇超声提取法获得各中药提取物,并以抗坏血酸(VitC)为阳性对照来考察其对超氧阴离子(O₂^-·)、羟自由基(·OH)、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的清除效果。 结果: 甘草、丹参、马尾松、当归、罗汉松对O₂^-·的抑制率分别为(浓度相当于原药材100 g·L^-1):15.86%,11.68%,11.31%,7.88%,5.94%;在清除DPPH·的实验中各中药的IC₅₀(g·L^-1)分别为:丹参1.6,马尾松1.87,罗汉松3.11,甘草13.91,当归22.32;而在清除·OH的实验中各中药的IC₅₀(g·L^-1)则为:丹参1.6,罗汉松2.02,马尾松2.1,甘草2.77,当归4.03;而三大体系阳性对照的IC₅₀分别为0.047,0.93,0.21 g·L^-1。 结论: 5种中药乙醇超声提取物均具有一定的抗氧化活性,其中丹参、马尾松效果较好;但与阳性对照Vit C相比仍有一定差距。

Antioxidant Activities of Ethanol Extracts from Five Kinds of Chinese Herbal Medicine

Objective: To study the preliminary antioxidant activities of ethanolextracts from several Chinese herbal medicine. Method: The in vitro scavenging activities of ethanol extracts by ultrasound on superoxide radical,hydroxyl radical and DPPH·were investigated with vitamin C(VC) as the positive control in this study. Result: The extracts of Glycyrrhiza uralensis, Salvia miltiorrhiza, Pinus massoniana, Angelica sinensis, Podocarpus macrophyllus had scavenging activities on superoxide radical(equivalent of raw material 100 g·L^-1,the eliminate rate was 15.86%,11.68%,11.31%,7.88%,5.94%,respectively), DPPH·(IC₅₀ value was 13.91,1.6, 1.87, 22.32, 3.11 g·L^-1, respectively),·OH(IC₅₀ value was 2.77, 1.6, 2.1, 4.03, 2.02 g·L^-1, respectively). The VC IC₅₀ value of the three experiments was 0.047, 0.93, 0.21 g·L^-1. Conclusion: Five kinds of ethanol ultrasonic extract from Chinese herbal medicine have antioxidant activity, especially S. miltiorrhiza and P. massoniana, which havs the value of exploiting and utilizing.