双秦眼用凝胶对家兔眼部刺激性及抗单疱病毒性角膜炎作用

目的: 观察双秦眼用凝胶对实验性兔单纯疱疹病毒性角膜炎的治疗作用、眼部刺激性以及在眼部的滞留时间。 方法: 采用单纯疱疹病毒(HSV-1)型 SM44毒株接种划伤角膜的方法建立单疱病毒性角膜炎模型,将造模成功的50只新西兰兔随机分为5组,每组10只,分别为10%,20%,30%的双秦眼用凝胶组,阿昔洛韦眼液组,凝胶基质组,各组予以相应滴眼剂点眼治疗,阿昔洛韦组每天5次,其余4组每天4次。采用裂隙灯显微镜观察每组角膜上皮病变、角膜基质病变及动物死亡率;对最佳作用浓度的双秦凝胶进行眼刺激性试验评价,并采用荧光示踪法观察其在眼部的滞留时间。 结果: 治疗7 d时,阿昔洛韦滴眼液及30%双秦眼用凝胶对角膜上皮病变及角膜实质性病变的治疗作用均明显优于凝胶基质组,10%及20%双秦眼用凝胶组,各组之间病变程度差异有统计学意义(P<0.05),30%双秦眼用凝胶组与阿昔洛韦眼液组无统计学差异。治疗后14 d,30%双秦眼用凝胶组的死亡率最低,但与其他组相比,差异无统计学意义。眼部刺激性试验结果显示30%双秦眼用凝胶点眼1 h后及点眼7 d后均无刺激性,与生理盐水、阿昔洛韦眼液相比, 眼刺激分值无统计学差异;30%双秦眼用凝胶结膜囊滞留时间(133±22.5) min和角膜滞留时间(30±7.8) min,明显长于阿昔洛韦滴眼液及生理盐水组(P<0.05)。 结论: 30%双秦眼用凝胶对家兔单纯疱疹性角膜炎有较好的治疗作用,其在眼部滞留时间较阿昔洛韦眼液明显延长,且无眼部刺激性,具有很好的开发前景。

Eye Irritation and Anti-herpes Simplex Keratitis Effect of Shuangqin Ophthalmic Gel in Rabbit

Objective: To observe eye irritation, and therapeutic effect of Shuangqin ophthalmic gel on experimental rabbit herpes simplex keratitis (HSK), as well as evaluate the ocular residence time of Shuangqin ophthalmic gel. Method: The experimental HSK was induced by inoculation of HSV-1 (SM44 strain) on the scratched cornea. Figty rabbits with HSK were divided into 5 groups:10% Shuangqin group, 20% Shuangqin group, 30% Shuangqin group, acyclovir eye drop group, and gel substrate group. Acyclovir eye drop group was treated 5 times a day, while the other groups were treated 4 times a day. The severity of corneal epithelial lesion and stromal lesion were graded and the mortality rates of the different groups were recorded. Eye irritation of the Shuangqin ophthalmic gel with the best therapeutic effect was evaluated by irritation experiment, and the retention time of Shuangqin ophthalmic gel was measured by fluorescence tracer method. Result: On the 7th day after treatment, ACV eye drop and Shuangqin ophthalmic gel had a better therapeutic effect on both epithelial HSK and stromal HSK than other groups, the severity scores in the five groups were significantly different (P<0.05). There was no significant difference between 30% Shuangqin ophthalmic gel group and ACV group. On the 14th day after treatment, the mortality rate was lowest in the Shuangqin ophthalmic gel group, but there was no significant difference among the groups. The result of irritation test showed 30% Shuangqin ophthalmic gel both had no eye irritation after treatment for one hour and 7 days. The irritation scores in the Shuangqin ophthalmic gel group, ACV group and physiological saline group were not significantly different. The residence time of Shuangqin ophthalmic gel was (133±22.5) min and (54.13±8.48)min in the conjunctival sac and cornea respectively, there was significant difference compared with ACV group and physiological saline group(P<0.05). Conclusion: 30% Shaungqin ophthalmic gel has a good therapeutic effect on experimental HSK, a prolonged residence time and no eye irritation, therefore it is a very good new drug candidate for HSK.