高天然本底辐射地区居民外周血淋巴细胞染色体稳定性畸变研究

目的　研究高天然本底辐射对居民外周血淋巴细胞染色体的稳定性畸变的影响。方法　分离淋巴细胞培养法制备染色体标本。用生物素标记的人全染色体探针 1号、2号和 4号进行荧光原位杂交 (FISH)的方法 ,对高天然本底辐射 (高本底 )地区的 31名受检者和对照地区的 2 9名受检者的外周血淋巴细胞染色作稳定性畸变 (易位 )进行分析。结果　高本底地区和对照地区的老年人或儿童的染色体易位率差异无显著性 (P >0 0 5 )。另外 ,我们可以看到染色体易位率和年龄的依赖关系 (P <0 0 1,rs=0 388,自由度 5 6 )。结论　高本底地区和对照地区的老年人或儿童的染色体易位率统计学上没有差异 ,高本底地区的高天然本底辐射对诱发的染色体稳定性畸变 (易位 )的贡献并不比其他如化学诱变剂或 (和 )自身代谢因素诱发稳定性畸变的贡献大。

Cytogenetic study of stable chromosome aberrations in residents of a high background radiation area in China

Objective To investigate the effect of high background radiation on the induction of stable chromosome aberrations. Methods By fluorescence in situ hybridization(FISH) technique chromosome Nos.1,2 and 4 were painted using specific biotin-labeled whole chromosome painting probes.Peripheral blood specimens were taken from 31 individuals living in the high background radiation area(HRBA) and 29 individuals in the control area. Results No significant difference was found in the frequencies of translocations between HBRA and the control ( P >0 05,Mann-Whitney U test) for both children and elderly individuals.On the other hand,correlation between age and translocation frequencies was significant at the 1% level( r-s =0.388 with 56DF). Conclusion There are no differences in the frequencies of translocations between HBRA and the control area.The present result indicates that the contribution of an elevated level of natural radiation in HBRA in China to the induction rate of stable type aberrations(translocations)dose not have a significant effect compared with the contributions from all other sources such as chemical mutagens and(or) metabolic factors.