紫外线照射充氧自血回输对梭曼中毒家兔氧自由基代谢的影响

目的 探讨紫外线照射充氧自血回输（UBIO）对家兔急性梭曼中毒后氧自由基代谢的影响。方法 将家兔100只随机分为5组，即正常对照组、中毒组、常规治疗组、UBIO治疗组及复合治疗组，每组20只。各组家兔于14d后检测血清中丙二醛（MDA）含量、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPx）及过氧化氢酶（CAT）活性。结果 与正常对照组相比，家兔梭曼中毒后，中毒组血清MDA含量及CAT活性明显升高（P〈0．01），SOD、GSH-Px活性明显降低（P〈0．05）。但经UBIO或复合治疗后，与中毒组比较，UBIO组及复合治疗组血清MDA含量明显降低（P〈0．01），SOD、GSH-Px及CAT活性明显升高（P〈0．05）。结论 家兔梭曼急性中毒后可伴有明显的氧自由基损伤，UBIO能明显提高机体抗氧化损伤能力，可以应用于梭曼急性中毒的治疗。

The influence of ultraviolet blood irradiation and oxygenation on oxygen free radical metabolism in rabbits with soman intoxication

Objective To investigate the effect of ultraviolet blood irradiation and oxygenation(UBIO) on the metabolism of oxygen free radicals in rabbits with acute soman intoxication. Methods One hundred rabbits were randomly divided into five groups: a control group, intoxication group, routine therapy group, UBIO therapy group and complex therapy group, and treated accordingly. After 14d, the concentration of malondiadehyde(MDA) and the activity of superoxide dismutase(SOD), glutathionperoxidase(GSH-Px) and catalase(CAT) in their blood serum were determined. Results The concentration of MDA and the activity of CAT in the intoxication group were significantly higher than in the controls, and the SOD and GSH-Px activities were significantly lower. After UBIO or complex therapy, serum MDA level was significantly lower than in the intoxication group, but the concentrations of SOD, GSH-Px and CAT were significantly higher. Conclusions Acute soman intoxication is associated with a significant increase in oxygen free radical injury in rabbits. UBIO therapy can improve antioxidant activity and can be used to treat acute soman intoxication.