ClC-3氯通道对H_2O_2诱导的大鼠主动脉平滑肌细胞凋亡的影响

目的研究C lC-3氯通道蛋白过表达对H2O2诱导的大鼠主动脉平滑肌细胞凋亡的影响。方法蛋白免疫印迹法检测C lC-3蛋白表达;形态学方法、DNA琼脂糖电泳、MTT法和流式细胞仪观察和分析H2O2诱导的大鼠主动脉平滑肌细胞形态学改变、DNA断裂、细胞存活率、凋亡率及C lC-3蛋白过表达对其影响。结果C lC-3蛋白过表达减轻H2O2诱导的大鼠主动脉平滑肌细胞形态学改变、DNA断裂及细胞凋亡率,增加细胞存活。结论提示C lC-3氯通道蛋白过表达保护H2O2诱导的大鼠主动脉平滑肌细胞凋亡。

Effects of ClC-3 chloride channel on H2O2-induced apoptosis in rat aortic vascular smooth muscle cells

Aim To investigate the effects of ClC-3 protein on H_2O_2-induced apoptosis in rat aortic vascular smooth muscle cells.Methods Western blot was performed to detect the expression of ClC-3 protein.Morphological analysis,DNA agarose gel electrophoresis,MTT assay and FACS were performed to measure the morphological changes,DNA ladder,viability and apoptotic rate in rat aortic smooth muscle cells.Results Overexpression of ClC-3 protein in rat aortic smooth muscle cells prevents the apoptotic events induced by H_2O_2 including ultrastructural alteration,DNA ladder and the apoptotic cell death.Conclusion ClC-3 overexpression prevents H_2O_2-induced apoptosis in rat aortic smooth muscle cells.