《新乡医学院学报》稿约

<正>《新乡医学院学报》(Journal of Xinxiang Medical University)是新乡医学院主管、主办的综合性医药卫生类学术期刊,月刊,每月5日出版,国内外公开发行。国际标准连续出版物号:ISSN 1004-7239,国内统一连续出版物号:CN 41-1186/R,CODEN XYIXEQ,邮发代号:36-145。近几年来本刊取得多项荣誉,被教育部科学技术司评为中国高校优秀科技期刊奖,被教育部科技发展中心评为"中国科技论文在线优秀期刊"一等奖2次、二等奖1次,被中国科学技术信息研究所收录为中国科技核心期刊(中国科技论文统计源期刊)、中国

Candida albicans induced expression of NLRP3 in murine peritoneal macrophage through activating cathepsin B

Objective To investigate the role of nucleotide-binding oligomerization domain-leucine-rich repeats containing pyrin domain 3 （ NLRP3 ） in the production of pro-inflammatory eytokines induced by Candida albicans in routine peritoneal macrophages and the activation pathway of NLRP3. Methods Murine peritoneal macrophages were cultured in vitro and random- ly divided into five groups： control group, CA-O74Me group, Candida albicans group, Candida albicans ＋ 10 -6 mol · L-1 CA- 074Me group and Candida albicans ＋ 10 -Smol · L -1 CA-074Me group. The mRNA expressions of NLRP3 in the cells were deter- mined by real-time fluorescence quantitative polymerase chain reaction. Western blot was preformed to detected the protein expression of cathepsin B and caspase-1 p20 in cytoplasm. The protein of intedeukin-1β（ IL-1β）was determined by enzyme-linked immunosorbent assay. Results Compared with control group, administration of Candida albicans not only enhanced the mRNA expressions of NLRP3 （P 〈 0. 01 ） ,but also elevated the protein expression of cathepsin B and caspase-1 p20 in cytoplasm（ P 〈 0. 01 ）. Murine peritoneal macrophages stimulated with Canclida albicans secreted significantly higher concentration of IL-1β than control group（ P 〈 0. 01 ）. After adding 10-5 mol · L- 1 CA-074Me into the cells challenged with Candida albicans ,the mRNA expressions of NLRP3,the activities of cathepsin B and caspase-1 p20 in macrophages and the levels of IL-113 in the supernatant decreased significantly than those in the Candida albicans group（P 〈0.01 ）, Conclusion Candida albicans induces the produc- tion of NLRP3 ,the activation of caspase-1 and pro-inflammatory cytokines IL-1β in macrophages via activating cathepsin B.