硫化氢和胱硫醚β合酶在脑室周围白质软化新生大鼠脑组织中的表达

目的探讨缺氧缺血对3日龄大鼠脑白质细胞硫化氢(H2S)及胱硫醚β合酶(CBS)表达的影响,以及其在3日龄大鼠脑白质损伤(WMD)发病过程中的作用。方法将3日龄大鼠按照随机数字表法分为实验组和对照组,建立新生大鼠WMD模型,分别在缺氧缺血后第12、24、48、72 h和7 d处死大鼠,脑组织取材后行苏木精-伊红(HE)染色,并观察其病理变化。采取免疫组织化学法检测脑组织CBS的表达水平;用分光光度计法测大鼠血浆中H2S水平。结果在新生大鼠脑白质中,缺氧缺血后实验组大鼠脑组织CBS的表达和血浆H2S水平在12 h开始增强,24 h达高峰,7 d仍可见表达;实验组大鼠脑组织CBS的表达和血浆H2S水平在缺氧缺血后12、24、48、72 h和7 d均高于对照组,差异有统计学意义(P<0.05)。结论新生大鼠脑组织CBS和血浆H2S表达水平于缺氧缺血后显著增强,二者可能与新生大鼠WMD的病理生理过程有关。

Expression of hydrogen sulphide and cystathionine-beta-synthase in immature rats with periventricu- lar leukomalacia

Objective To observe the effect of hypoxia-ischemia （HI） on expressions of hydrogen sulfide （ H2 S） and cystathionine-beta-synthase（CBS） protein in alba of 3 days old rats, and to explore the possible role of them in pathogenesis of white matter damage （WMD）. Methods The 3 days old rats were randomly divided into experimental group and control group. The model of WMD was established ,then the rats were sacrificed at 12,24,48,72 hours and 7 days after HI. The pathological change of brain tissue was observed by haematoxylin-eosin. Immunohistochemical technic was applied to investigate the change of CBS expression of in the brain tissue of the rats. The plasma level of Ha S was detected by the spectrophotometer. Results In the experimental group, the expression of CBS in the brain tissue of 3 days old rats and the level of H2 S increased at 12 hours and reached the peak at 24 hours after HI,which still could be found 7 days after HI. The expression of CBS in the brain tissue of 3 days old rats and the level of H2S at 12,24,48,72 hours and 7 days after HI in experimental group were higher than those in control group, there were significant differences between the two groups （ P 〈 0.05 ）. Conclusion The expressions of CBS and H2 S in the brain tissue of 3 days old rats increase significantly after HI, and they may be involved in the pathophysiological process in the WMD.