| TLCS同时测定复方补乌糖浆中3种指标成分含量 |
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| 引用本文： | 陈莉,王盛,吴溪,等.TLCS同时测定复方补乌糖浆中3种指标成分含量[J].中国中医药信息杂志,2014(2):92-94. |
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| 作者姓名： | 陈莉 王盛 吴溪 等 |
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| 作者单位： | [1]安徽中医药大学第一附属医院,安徽合肥230031 [2]国家中医药管理局中药制剂三级实验室,安徽合肥230031 |
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| 基金项目： | 基金项目：安徽中医药大学青年科研基金（2012qn015） |
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| 摘 要： | 目的建立同时测定复方补乌糖浆中3种成分含量的方法。方法采用薄层色谱扫描法(TLCS),硅胶G薄层板,环己烷-乙酸乙酯-二氯甲烷-甲酸(3∶1∶1∶0.2)为展开剂,检测波长λS=300 nm。结果阿魏酸在0.36~0.84μg、补骨脂素在0.12~0.28μg、大黄素在0.01~0.05μg线性范围内呈良好的线性关系,平均回收率分别为100.7%、100.8%、101.0%,RSD分别为1.26%、1.44%、1.86%。结论该方法简单、快速、高效,稳定可靠,可作为复方补乌糖浆的质量控制方法。
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| 关 键 词： | 复方补乌糖浆 薄层色谱扫描法 含量测定 |
Simultaneous Determination of Three Marker Components in Fufang Buwu Syrup by TLCS |
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| CHEN Li,WANG Sheng,WU Xi,MENG Mei,ZHU Jing-jing.Simultaneous Determination of Three Marker Components in Fufang Buwu Syrup by TLCS[J].Chinese Journal of Information on Traditional Chinese Medicine,2014(2):92-94. |
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| Authors: | CHEN Li WANG Sheng WU Xi MENG Mei ZHU Jing-jing |
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| Institution: | 1.The First Affiliated Hospital of Anhui University of TCM, Hefei 230031, China; 2.State Administration of National Chinese Drugs Aledicament Laboratory of Grade 3, Hefei 230031, China) |
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| Abstract: | Objective To establish an accurate method for determining the content of three components in Fufang Buwu Syrup. Methods TLC scanner was selected to detect three components with silica gel G thin layer plate. The sample was separated by using cyclohexane-ethyl acetate-methylenechloride-formic acid （3 ： 1 ： 1 ： 0.2）,λs=300 nm. Results The linearity between peak area and ferulie acid was achieved in the range of 0.36-0.84 μg, psoralen was achieved in the range of 0.12-0.28μg, emodin was achieved in the range of 0.01-0.05μg. The average recovery was 100.7%, 100.8%, 101.0%, and RSD was 1.26%, 1.44%, 1.86%, respectively. Conclusion The method is simple and accurate, which can be used for quality control of Fufang Buwu Syrup. |
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| Keywords: | Fufang Buwu Syrup TLCS content determination |
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