| miR-223-3p靶向调控Rbpj转录因子对葡萄膜炎大鼠Th1和Th17细胞分化的影响 |
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| 引用本文： | 周梦贤,屈如意,殷学伟,郭励劼,邱岩,郭大东. miR-223-3p靶向调控Rbpj转录因子对葡萄膜炎大鼠Th1和Th17细胞分化的影响[J]. 国际眼科杂志, 2022, 22(5): 769-774 |
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| 作者姓名： | 周梦贤 屈如意 殷学伟 郭励劼 邱岩 郭大东 |
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| 作者单位： | 中国山东省济南市,山东中医药大学,中国山东省济南市,山东中医药大学,中国山东省济南市,山东中医药大学,中国北京市,首都医科大学,中国山东省济南市,山东中医药大学第二附属医院,中国山东省济南市,山东省中西医结合眼病防治重点实验室 山东省高校中西医结合眼病防治技术(强化)重点实验室 山东中医药大学眼科研究所 |
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| 基金项目： | 国家自然科学基金(No.81873163); 山东省自然科学基金项目(No.ZR2020KC024) |
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| 摘 要： | 目的:探讨miR-223-3p对Notch信号通路转录因子Rbpj表达的调控作用以及对实验性自身免疫性葡萄膜炎(EAU)大鼠Th1、Th17细胞分化水平的影响。方法:用双荧光素酶表达报告系统探讨miR-223-3p对Rbpj基因表达的调控作用。将24只雌性Lewis大鼠随机分为EAU模型组、正常对照组(NC组)和空白对照组(BC组),每组8只。EAU模型组注射含光感受器间维生素A结合蛋白(IRBP)、结核菌素和完全弗氏佐剂的乳糜液以诱导葡萄膜炎，BC组注射等体积的不含IRBP多肽的乳糜液，NC组注射等体积的无菌生理盐水。免疫后12d,无菌分离三组大鼠的脾脏、淋巴结和眼组织，实时荧光定量PCR(Q-PCR)检测miR-223-3p和Rbpj基因的表达水平；酶联免疫吸附测定(ELISA)方法检测Rbpj、IFN-γ、IL-17蛋白的表达水平；流式细胞仪检测三组大鼠不同组织中Th1和Th17细胞的表达水平。结果:双荧光素酶检测结果证实Rbpj是miR-223-3p调控的靶基因。免疫后12d,相比于NC组，EAU模型组大鼠脾脏、淋巴结和眼组织中miR-223-3p相对表达水平分别为0.33±0...
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| 关 键 词： | miR-223-3p Rbpj 实验性自身免疫性葡萄膜炎 Th1 Th17 IFN-γ IL-17 |
| 收稿时间： | 2021-06-04 |
| 修稿时间： | 2022-03-31 |
Study on the effect of miR-223-3p regulating transcription factor Rbpj on the differentiation of Th1 and Th17 cells in rats with experimental autoimmune uveitis |
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| Meng-Xian Zhou,Ru-Yi Qu,Xue-Wei Yin,Li-Jie Guo,Yan Qiu and Da-Dong Guo. Study on the effect of miR-223-3p regulating transcription factor Rbpj on the differentiation of Th1 and Th17 cells in rats with experimental autoimmune uveitis[J]. International Eye Science, 2022, 22(5): 769-774 |
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| Authors: | Meng-Xian Zhou Ru-Yi Qu Xue-Wei Yin Li-Jie Guo Yan Qiu Da-Dong Guo |
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| Affiliation: | Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China,Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China,Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China,Capital Medical University, Beijing 100069, China,The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250002, Shandong Province, China and Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases;Key Laboratory of Integrated Traditional Chinese and Western Medicine Eye Disease Prevention and Treatment Technology(Enhanced)in Universities of Shandong Province;Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan 250002, Shandong Province, China |
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