| 腺病毒介导的HCY2基因对血管平滑肌细胞凋亡的诱导作用 |
| |
| 引用本文： | 李黔,郭艳红,曲明娟,陈光慧,高炜. 腺病毒介导的HCY2基因对血管平滑肌细胞凋亡的诱导作用[J]. 中国介入心脏病学杂志, 2003, 11(2): 96-98 |
| |
| 作者姓名： | 李黔 郭艳红 曲明娟 陈光慧 高炜 |
| |
| 作者单位： | 100034,北京大学第一医院心内科 |
| |
| 基金项目： | 北京市自然基金资助项目 (bjbd -19993 11) |
| |
| 摘 要： | 目的 观察高同型半胱氨酸诱导基因HCY2对于平滑肌细胞的作用。方法 以复制缺陷型腺病毒作为载体 ,将HCY2基因转移到平滑肌细胞中。提取平滑肌细胞的DNA ,进行凝胶电泳及ELISA ,行DNA片段化分析。用流式细胞术观察平滑肌细胞的亚二倍体。结果 转染HCY2后平滑肌细胞的DNA断裂成相差 2 0 0bp左右的片段 ,流式细胞术测定时发现 ,位于亚二倍体区的平滑肌细胞明显增多。结论 HCY2基因能引起平滑肌细胞的凋亡。
|
| 关 键 词： | 平滑肌细胞 凋亡 腺病毒 |
| 修稿时间： | 2003-01-21 |
Adenovirus-mediated HCY2 induces apoptosis in vascular smooth muscle cells |
| |
| LI Qian,GUO Yanhong,QU Mingjuang,et al. Adenovirus-mediated HCY2 induces apoptosis in vascular smooth muscle cells[J]. Chinese Journal of Interventional Cardiology, 2003, 11(2): 96-98 |
| |
| Authors: | LI Qian GUO Yanhong QU Mingjuang et al |
| |
| Affiliation: | LI Qian,GUO Yanhong,QU Mingjuang,et al Department of Cardiology,Peking University First Hospital,Beijing 100034,China |
| |
| Abstract: | Objective To investigate roles of Homocysteine induced gene HCY2 in vascular smooth muscle cells (VSMC) Methods HCY2 was delivered into VSMC by replication defective adenovirus DNA fragmentation was investigated by DNA Laddering and cell death ELISA VSMC in sub G1 was detected by using flow cytometry Results DNA from VSMC transfected by HCY2 gene was cleaved into multiple nucleosomes and VSMC in sub G1 region increased after being infected with HCY2 Conclusion HCY2 is able to induce apoptosis in VSMC |
| |
| Keywords: | Smooth muscle cell Apoptosis Adenovirus |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
