慢病毒介导大鼠卵巢基因转染的实验研究 *

目的 研究活体大鼠卵巢中慢病毒载体介导的基因转染及表达情况。方法 慢病毒载体(携带GFP的Bcl-2-HIV-I)注射大鼠卵巢实质，分别于注射后1、3、7、14、28天处死大鼠（每个时间点实验组n=2，对照组n=2，空白对照组n=2），通过 Western-blot 电泳检测报告基因的表达。结果 实验组大鼠可见Bcl-2基因的表达，明显高于对照组和空白对照组Bcl-2基因的表达。 结论 慢病毒能有效的将外源基因导入活体大鼠卵巢而无明显毒副作用。

Experimental study of lentivirus-mediated gene transduction in rat ovarian

Objective To analyze gene transduction of rat ovarian in vivo after administration of lentiviral vectors. Methods The Bcl-2-HIV-I carrying GFP were delivered into the ovarian parenchyma.Animals were killed at 1, 3,7,14 and 28 days after treatment( 2 cases each time point for experimental animals , 2 cases each time point for controls and 2 cases each time point for blank controls). The transducted gene expression was determined by Western-blot electrophoresis. Results Bcl-2 gene were detectable in the ovarians injected with the lentiviral vector and were significantly higher than in the contralateral ovarians and blank contralateral ovarians .Conclusion Lentiviral vector can effectively and stably transfer exogenous gene to rat ovarians in vivo without significant toxic effects.