中枢神经系统炎症患者脑脊液S-100Β蛋白和神经元特异性烯醇化酶含量的测定

为了探讨脑脊液(CSF)S-100Β蛋白和神经元特异性烯醇化酶(NSE)含量对中枢神经系统(CNS)炎症患者脑损伤的评估价值,采用酶联免疫吸附试验双抗体夹心法对42例单纯疱疹病毒性脑炎(病脑组)、19例化脓性脑膜炎(化脑组)、17例隐球菌性脑膜炎(隐脑组)患者和22例无神经系统疾病、无肿瘤的外科腰麻患者(对照组)CSF中的S-100Β蛋白和NSE进行了动态观察.结果显示,3组CNS炎症患者脑脊液 S-100Β蛋白含量均显著高于对照组(P＜0.01),其中病脑组＞化脑组＞隐脑组(P＜0.01);脑脊液NSE含量病脑组最高,与化脑组和隐脑组相比差异有著性意义(P＜0.05和P＜0.01),隐脑组与对照组差异无显著性意义(P＞0.05);动态检测发现,脑脊液S-100Β蛋白和NSE含量随3组CNS炎症患者病情的轻重而发生相应的变化.研究表明,3组CNS炎症患者脑脊液 S-100Β和NSE含量均有不同程度的增高,且与患者脑损伤的严重程度有关.

DETERMINATION OF S-100 BETA PROTEIN AND NEURON-SPECIFIC ENOLASE OF CEREBROSPINAL FLUID IN PATIENTS WITH CENTRAL NERVOUS SYSTEM INFECTIONS

In order to explore the value of assessment in measuring S-100 beta protein (S-100B protein) and neuron-specific enolase (NSE) present in cerebrospinal fluid (CSF) from patients with brain insult in central nervous system infections, S-100B protein and NSE in CSF were measured dynamically by ELISA in 42 patients with Herpes Simplex Encephalitis (USE), 19 patients with purulent meningitis, 17 patients with cryptococcus meningitis and 22 unrelated control patients. It was found that the levels of CSF S-100B protein in all the three groups were higher than that of the control group (P<0. 01). The level of CSF NSE in HSE group was higher than that in the other three groups (P0. 05). There were dynamic changes of CSF S-100B protein and NSE concentrations in all three groups correlating with the severity of disease. It is suggested that elevated concentrations of S-100B protein and NSE in CSF related to the damage of glia and neurons could be used as CSF markers of brain insult in patients with central nervous system infections.