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| 放射性粒子125I对胶质瘤细胞治疗作用的实验研究 | |
| 引用本文： | 潘宝根,赵文清,李文玲,王跃红,边艳珠. 放射性粒子125I对胶质瘤细胞治疗作用的实验研究[J]. 第二军医大学学报, 2010, 31(1): 46-50. DOI: 10.3724/SP.J.1008.2010.00046 |
| 作者姓名： | 潘宝根 赵文清 李文玲 王跃红 边艳珠 |
| 作者单位： | 1. 河北医科大学外科教研室,石家庄,050031 2. 河北医科大学外科教研室,石家庄,050031;河北省人民医院神经外科,石家庄,050051 3. 河北省人民医院神经外科,石家庄,050051 4. 河北省人民医院核医学科,石家庄,050051 |
| 基金项目： | 河北省自然科学基金(C2004000671)~~ |
| 摘 要： | 目的探讨放射性粒子125I在动物体内抗人脑胶质瘤的效果及其作用机制。方法建立人胶质瘤的动物模型,瘤内植入放射性粒子125I,观察肿瘤生长情况,在电镜下观察其超微结构的变化,采用TUNEL技术检测肿瘤组织的凋亡情况,流式细胞仪检测Bax/Bcl-2的表达,并应用免疫组化染色检测血管密度和VEGF、bFGF的表达。结果成功建立了人胶质瘤细胞U251的动物模型,植入放射性粒子125I后,胶质瘤生长较对照组明显受到抑制(P<0.05,P<0.01),电镜下可呈现核断裂、染色质边聚等细胞凋亡的形态特征。检测显示:和对照组相比,肿瘤组织的Bax/Bcl-2(1.88±0.47vs1.11±0.52,P<0.01)和组织凋亡率的IHS评分(3.64±0.89vs0.81±0.45,P<0.01)明显增高;肿瘤组织的微血管密度IHS评分(1.40±0.55vs3.23±0.87,P<0.01)、VEGF的IHS评分(1.58±0.55vs4.19±0.45,P<0.01)和bFGF的IHS评分(2.44±0.89vs3.52±0.79,P<0.05)明显降低。结论放射性粒子125I对在体人胶质瘤U251有明确的抑制作用,促进... |
| 关 键 词： | 胶质瘤 间质内放疗 放射性核素125I 动物模型 细胞凋亡 微血管密度 |
| 收稿时间： | 2008-09-17 |
| 修稿时间： | 2009-12-06 |
Therapeutic effect of 125 I in treatment of nude mice implanted with human glioma |
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| PAN Bao-gen,ZHAO Wen-qing,LI Wen-ling,WANG Yue-hong,BIAN Yan-zhu. Therapeutic effect of 125 I in treatment of nude mice implanted with human glioma[J]. Former Academic Journal of Second Military Medical University, 2010, 31(1): 46-50. DOI: 10.3724/SP.J.1008.2010.00046 | |
| Authors: | PAN Bao-gen ZHAO Wen-qing LI Wen-ling WANG Yue-hong BIAN Yan-zhu |
| Affiliation: | PAN Bao -gen1,ZHAO Wen-qing1,2,LI Wen-ling2,WANG Yue-hong1,BIAN Yan-zhu3 1. Department of Surgery,Hebei Medical University,Shijiazhuang 050031,Hebei,China 2. Department of Neurosurgery,Hebei General Hospital,Shijiazhuang 050051,China 3. Department of Nuclear Medicine,China |
| Abstract: | Objective To investigate the efficacy of 125I in treatment of nude mice implanted with human glioma cell line U251 and the related mechanism. Methods The animal models of human malignant glioma were established with nude mice. 125I was implanted into tumors and the tumor growth was observed. The ultrastructural change was observed by electron microscopy. The apoptosis of the tumor tissue was examined by in situ end -labelling technique; expression of Bax/Bcl -2 was detected by flow cytometer. Blood vessel d... |
| Keywords: | glioma brachytherapy radioisotope 125I animal model apoptosis microvessel density |
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