| ltB-TpN47融合基因原核表达系统的构建 |
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| 引用本文： | 宋春涵,何旭瑛,高素华. ltB-TpN47融合基因原核表达系统的构建[J]. 江西医学检验, 2008, 26(1): 27-29 |
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| 作者姓名： | 宋春涵 何旭瑛 高素华 |
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| 作者单位： | 宋春涵（321017,浙江省金华职业技术学院医学院免疫学教研室）;何旭瑛（321017,浙江省金华职业技术学院医学院免疫学教研室）;高素华（321017,浙江省金华职业技术学院医学院免疫学教研室） |
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| 摘 要： | 目的分别从梅毒螺旋体（Treponema pallidum,Tp）临床菌株和大肠杆菌DNA中扩增ltB和TpN47基因,构建ltB-TpN47融合基因及其原核表达系统。方法采用PCR分别从上述菌株DNA中扩增TpN47基因和ltB基因片段、构建ltB-TpN47融合基因,T-A克隆后测定核苷酸序列。采用质粒pET32和宿主菌E.coliBL21DE3构建ltB-TpN47融合基因原核表达系统,并用不同浓度的IPTG诱导表达。结果所构建的ltB-TpN47融合基因核苷酸序列与从GeneBank中查询并处理得出的数据基本一致。所构建的表达系统pET32-ltB-TpN47的目的重组蛋白（rltB-TpN47）表达量高达细菌总蛋白的1/3左右。结论本文成功地构建了ltB-TpN47融合基因高效原核表达系统。
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| 关 键 词： | 梅毒螺旋体/TpN47基因 大肠杆菌/ltB基因 融合基因 克隆/表达 |
| 文章编号： | 1674-1129(2008)01-0027-03 |
The construction of prokaryotic expression product of ltB-TpN47 fusion gene |
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| SONG Chunhan,HE Xuying,GAO Suhua. The construction of prokaryotic expression product of ltB-TpN47 fusion gene[J]. Jiangxi Journal of Medical Laboratory Sciences, 2008, 26(1): 27-29 |
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| Authors: | SONG Chunhan HE Xuying GAO Suhua |
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| Affiliation: | SONG Chunhan,HE Xuying,GAO Suhua. Department of Immunology,the School of Medicine,JinHua College Of Profession & Technology,Jinhua 321017,China |
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| Abstract: | Objective To amplify ltB gene and TpN47 gene from DNAs of a clinical strain Treponema pallidum and Escherichia coli strain,respectively,and construct ltB-TpN47 fusion gene and its prokaryotic expression system. Methods TpN47 and ltB genes from DNAs of the bacteria mentioned above were amplified and ltB-TpN47 fusion gene was constructed by PCR. Amplification fragments of the target genes were sequenced after T-A cloning. Plasmid pET32 and E.coli strain BL21 DE3 were applied to construct expression system of ... |
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| Keywords: | Treponema pallidum / TpN47 gene Escherichia coli / ltB gene fusion gene Cloning / expression |
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