BEP2D细胞和R15Hp35T-2细胞中60个肺癌相关基因的表达研究

目的：研究永生化人支气管上皮细胞（BEP2D）和α粒子辐射诱发BEP2D细胞后形成的恶性转化细胞（R15Hp35T-2)中60个肺癌相关基因的表达谱。方法：首先搜集了60个肺癌相关的基因，经扩增和纯化后，用Cartesian PixSys550 cDNA Microarray点样仪将60个肺癌相关基因以微阵列形式点布于醛基化的玻璃片上。然后提取BEP2D细胞和R15Hp35T-2细胞的RNA，经长片段反转录和线性扩增标记成荧光探针后与微阵列中的cDNA进行杂交。结果：与BEP2D细胞相比，R15Hp35T-2细胞中上调表达的基因有27个，下调表达的基因有7个。大部分抑癌基因的mRNA丰度在2种细胞中表达相似；而大多数癌基因和生长因子类基因的mRNA丰度在R15Hp35T-2细胞中高表达。结论：在辐射诱发的人支气管上皮恶性转化细胞中，癌基因及生长因子类基因可能共同促进了细胞的转化。

Expression profile of 60 lung cancer related genes in BEP2D cell and R15Hp35T-2 cell

Objective To profile the expression patterns of 60 lung cancer related genes in human bronchial epithelial cell (BEP2D) and alpha-particle induced malignantly transformed cell (R15Hp35T-2). Methods Sixty lung cancer related cDNAs were micro-arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from BEP2D cell and R15Hp35T-2 cell was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA. Results Compared with the BEP2D cell, 27 genes up-regulated and 7 down-regulated in the R15Hp35T-2 cell. The expression abundance of most tumor suppressor genes were similar in the two kinds of cells, however, most oncogenes and growth factor genes were overexpressed in R15Hp35T-2 cell. Conclusion In malignantly transformed human bronchial epithelial cell model induced by alpha-particle, some oncogenes and growth factor genes may promote the malignant transformation together.