厄贝沙坦对早期糖尿病肾病大鼠足细胞nephrin及podocin表达的影响

目的：探讨厄贝沙坦对早期糖尿病肾病大鼠足细胞分子 nephrin、podocin mRNA表达的影响。方法：将36只Wistar大鼠随机分为3组：正常对照组（N组）、糖尿病肾病组 （DM组 ） 和厄贝沙坦干预组（E组）。E组大鼠给予厄贝沙坦50mg·kg^-1·d^-1 灌胃,N组和DM组大鼠给予等量饮用水灌胃。于第8周和第12周检测3组大鼠24h尿蛋白 （Pro） 定量、血清肌酐（Scr）水平;用RT-PCR方法检测肾皮质nephrin、podocin mRNA表达水平。结果：造模后第8周和第12周,DM组大鼠24hPro、Scr水平与N组比较,差别有统计学意义（P〈0.01）;造模后第8周和第12周,E组大鼠24h Pro与DM组比较,差别有统计学意义（P〈0.05）；造模后第12周Scr水平与DM组比较,差别有统计学意义（P〈0.05）。第8和第12周,DM组大鼠nephrin和podocin mRNA表达水平与 N组比较,差别有统计学意义（P〈0.01）;E组大鼠nephrin和podocin mRNA表达水平与DM组大鼠比较,差别亦有统计学意义（P〈0.05）。结论：厄贝沙坦能通过上调nephrin和 podocin表达水平来改善足细胞功能,减少糖尿病肾病大鼠蛋白尿。

Of Irbesartan on early diabetic nephropathy podocyte molecules nephrin,podocin mRNA expression inEffects of irbesartan on expression of rat podocyte nephrin and podocin in early diabetic nephropathy

Objective.To investigate the effects of irbesartan on expression of podocyte nephrin and podocin mRNA in early diabetic nephropathy rats. Methods ：Thirty-six Wistar rats were divided into three groups： normal group,diabetic nephropathy group and irbesartan intervention group. The Irbesartan intervention group was given irbesartan 50mg·kg^-1·d^-1 for gavage and the normal control group and diabetic nephropathy group were given equal amount of drinking water for gavage,The 24h urinary protein. Results：At 8 and 12 weeks after the models were prepared,there was a difference in the levels of 24h urinary protein and serum creatinine between the diabetic nephropathy group and normal group （P 〈 0.01）, and in the 24 h urinary protein level between the Irbesartan intervention group and the diabetic nephropathy group （P 〈 0. 05） and in the serum creatinine level between the Irbesartan intervention group and the diabetic nephropathy group at 12 weeks （P 〈 0.05）. There was a difference in the nephrin and podocin mRNA expression levels at 8 and 12 weeks between the diabetic nephropathy group and the normal group （P 〈 0. 01） and between the Irbesartan intervention group and the diabetic nephropathy group （P 〈 0.05）. Conclusion. Irbesartan can improve the podocyte function and reduce proteinuria in diabetic nephropathy by up regulation of the expression of nephrin and podocin.