p-mTOR、P-p70S6K蛋白在膀胱尿路上皮癌中的表达

目的：探讨哺乳动物雷帕霉素靶蛋白（mTOR）通路蛋白（p-mTOR）、核糖体蛋白s6激酶（p-p70S6K）在膀胱尿路上皮癌中的表达情况及与预后的关系。方法：利用免疫组化染色检测mTOR通路蛋白p-mTOR、P-p70S6K在膀胱尿路上皮癌中的表达情况，分析p-mTOR、P-p70S6K在不同膀胱组织中的表达差异，与膀胱癌临床病理参数的关系及与膀胱尿路上皮癌预后的关系。结果：p-mTOR在膀胱尿路上皮癌中的阳性表达率（43.3％）高于正常黏膜（17．5％），p-mTOR表达阳性率随着肿瘤分级分期的增加相应增加，多发性膀胱尿路上皮癌p-mTOR表达阳性率（70．5％）高于单发组（31．4％），复发组（70．5％）高于未复发组（31.4％，P〈0．01）；P-p70S6K在膀胱尿路上皮癌阳性率（25．9％）高于正常膀胱黏膜（7．5％，P〈0．05），p-p70S6K表达阳性率随着肿瘤分级分期的增加相应增加，多发性膀胱尿路上皮癌p-p70S6K表达阳性率（45．9％）高于单发组（17．1％），复发组（30．3％）高于未复发组（17．4％，P〈0．01）；肿瘤分级、分期、数目及p-mTOR表达分别是膀胱肿瘤无瘤生存的独立预后因子。结论：mTOR／p-mTOR／p-p70S6K通路的激活与膀胱尿路上皮癌的发生发展有关，p-mTOR可以作为预测膀胱尿路上皮癌复发的预后因子。

Expression of p-mTOR and p-p70S6K in Urothelial Bladder Cancer

Objective To investigate the expression of p-mTOR and p-p70S6K in human bladder cancer tissues and the correlation with the prognosis. Methods The expression of p-mTOR and p-p70S6K was de- tected in normal bladder tissues and bladder cancer tissues by immunohistochemistry. Their relationship with clinicopathological characteristics and prognosis was analysed by Kaplan-Meier method and Log rank test. Results The expression of p-roTOR in bladder cancer tissues was 43.3%, higher than 17.5% in normal blad- der tussues. The positive rate of p-mTOR expression increased with the progress of pathological stage and tumor grade. The expression of p-roTOR was 70.5% in multiple bladder tumors, higher than 31.4% in single bladder tumor; 70.5% with positive expression of p-mTOR relapsed, higher than 31.4% without recurrence. The expres- sion of p-p70S6K in bladder cancer tissues was 25.9%, higher than 7.5% in normal bladder tussues. The posi- tive rate of p-pTOS6K expression increased with the progress of pathological staging and tumor grade. The ex- pression of p-p70S6K was 45.9% in multiple bladder tumors than 17.1% in single bladder tumor; 30.3% with positive expression of p-p70S6K relapsed, higher than 17.4% without recurrence. The difference was statistical significant. The pathological staging, tissue grade, number of tumors and expression of p-roTOR were identified as independent prognostic factors for bladder cancer by univariate and multivariate Cox regression analysis. Conclusion The mTOR/p-mTOR/p-p70S6K pathway was correlated with tumor oncology and tumor progress, p-mTOR protein was an independent prognostic factor for bladder cancer.