氧化应激与脑卒中后痴呆转归的关系分析

目的:探讨氧化应激(OS)与脑卒中后认知障碍(PSCI)的关系。方法:按入组标准选择108例急性脑卒中患者,其中缺血性脑卒中62例,出血性脑卒中46例。50例同期门诊体检的老年人作为对照组。对所有入组患者在急性期(14 d)进行Mo CA评分,于发病3个月后对患者进行随访及采集血液标本保存待测。采用ELISA法检测血清中8-羟基脱氧鸟苷酸(8-OHd G)、3-硝基酪氨酸(3-NT)及8-异构前列腺素F2a(8-isoPGF2α),比色法检测总超氧化物岐化酶(T-SOD)。结果:发病3个月后神经心理学评分显示认知进一步恶化患者与认知改善患者比较,认知恶化组8-Ohd G、3-NT、8-iso-PGF2α表达高,T-SOD表达低(P0.05);发病3个月后,转化为血管性痴呆(VD)患者与未转化患者比较,转化为VD组8-Ohd G、3-NT、8-iso-PGF2α表达高,T-SOD表达低(P0.01)。结论:氧化应激与脑卒中后痴呆转化有关,有可能是脑卒中后认知障碍的独立危险因素。

Study of the Relationship of the transformation between Oxidative Stress and Post StroKe Dementia

Objective:To research the relationship of oxidatie stress and post stroke cognitive impairment. Methods:Selected 108 cases of acute stroke pa-tients,including 62 cases of cerebral ischemic stroke and 46 cases of Hemorrhagic stroke. 50 cases of old people in control group was selected from outpatient. To evaluate their cognition by montreal cognitive assessment(MoCA)score and collect blood samples in the acute phase,in 3 months. 8 - OhdG,3 - NT and 8 - iso- PGF2α were measured by enzyme linked immunosorbent assay(ELISA),while T - SOD was measured by photocolorimetric method. Results:3 months after stroke occurred,the expression of 8 - OhdG,3 - NT and 8 - iso - PGF2α in the deteriorated group was higher compared to those of the improved group,but the expression of T - SOD was lower(P ﹤ 0. 05). 3 months after stroke occurred,the expression of 8 - OhdG,3 - NT and8 - iso - PGF2α in serum of patients with VaD was higher than those with no VaD,but the expression of T - SOD was lower(P ﹤ 0. 01). Conclusions:0xidative stress are related to the transformation of dementia,is probablely to be an independent risk factor for post stroke cognitive impairment.