参芪泄浊饮对腺嘌呤肾间质纤维化模型大鼠肾组织MMP-9、TIMP-1基因表达的影响

目的:观察参芪泄浊饮对慢性肾衰大鼠肾组织基质金属蛋白酶9(MMP-9)、基质金属蛋白酶抑制剂1(TIMP-1)基因表达的影响。方法:采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、常规剂量组、氯沙坦组、模型组及空白组,高剂量组、常规剂量组、氯沙坦组分别予参芪泄浊饮水煎液20 m L/(kg·d)、参芪泄浊饮10 m L/(kg·d)、氯沙坦9 mg/(kg·d),模型组与空白组大鼠均予纯净水10 m L/(kg·d),连续给药8周,全部大鼠麻醉后腹腔采血,取左肾,ELLISA法测量血肌酐(Scr)、尿素氮(BUN)、丙氨酸转氨酶(ALT)并采用荧光定量PCR检测肾脏MMP-9、TIMP-1 mRNA的表达。结果:1高剂量组Scr水平明显低于常规剂量组、氯沙坦组及模型组;常规剂量组与氯沙坦组无明显差别,且均低于模型组;2高剂量组MMP-9 mRNA表达水平明显高于常规剂量组、氯沙坦组及模型组;常规剂量组与氯沙坦组无明显差别,且均高于模型组;高剂量组TIMP-1 mRNA表达水平明显低于常规剂量组、氯沙坦组及模型组;常规剂量组与氯沙坦组无明显差别,且均低于模型组;3各组ALT水平较空白组比较均无显著差别。结论:参芪泄浊饮可通过调节MMP-9及TIMP-1表达来缓解慢性肾衰肾脏功能的恶化。

Effects of Shenqi Xiezhuo Decoction on MMP-9 and TIMP-1 Expression in Adenine-induced Renal Interstitial Fibrosis Rats

Objective: To investigate the effects of Shenqi Xiezhuo Decoction on matrix metalloproteinase-9( MMP-9) and tissue inhibitor of matrix metalloproteinase-1( TIMP-1) expression in adenine-induced renal interstitial fibrosis rats. Methods:The renal failure model of rats was established by Yokozawa method. All rats were random Ly divided into 5 groups: high-dose group,regular-dose group,losartan group,model group and blank group. After 8 weeks,all rats' left kidney and blood were analyzed by RT-PCR and serum biochemical tests. Results: 1The level of serum creatinine of high-dose group was significantly lower than the levels of regular-dose group or losartan group and there was no significant difference between the latter two groups. 2The expression of MMP-9 of high-dose group was more than that of the regular-dose group or losartan group and there was no significant difference between the latter two groups. The expression of TIMP-1 of high-dose group was less than that of the regular-dose group or losartan group and there was no significant difference between the latter two groups. 3There was no difference of the level of ALT between blank group and the others. Conclusion: Shenqi Xiezhuo Decoction may alleviate deterioration of the renal function by regulating MMP-9 and TIMP-1 balance.