基质金属蛋白酶-9与宫颈成熟关系的研究

目的 研究分娩时宫颈组织中是否有基质金属蛋白酶-9（MMP-9）的合成，及其来源与分布。方法 以MMP-9单克隆抗体为一抗，用ABC法对分娩期孕妇宫颈组织（n=10）进行免疫组化分析，以非孕期宫颈组织作对照（n=7）。结果 分娩期孕妇宫颈中浸润的多形核白细胞（PMN）胞浆中有MMP-9的阳性染色，宫颈血管中的PMN胞浆中有阳性染色，宫颈组织间质中有散在弱阳性染色；其它细胞均无阳性染色，对照组中未发现MMP-9的阳性染色。结论 分娩时宫颈组织的MMP-9的合成和分泌，主要来源于宫颈中浸润的PMN；MMP-9可能是宫颈成熟过程中重要的调控因子。

Relationship of matrix metalloproteinase-9 and ripening of pregnant cervix

Objective To investigate whether matrix metalloproteinase 9 (MMP 9) was synthesized in pregnant cervix during parturition and its source and distribution. Methods Cervical species ( n =10, each weighing about 0.3 g) were taken from pregnant women immediately after delivery . Other cervical species ( n =7) were served as negative control from those non pregnant women but undergoing uterotomy due to other benign diseases. Immunohistochemical method (ABC) was carried out to detect the expression of MMP 9, with a monoclonal antibody against MMP 9. Results Positive staining of MMP 9 was found in the cytoplasm of polymorphonuclear leukocytes (PMN) that had infiltrated into cervix or located in blood vessels of cervix. Scattered light positive staining were found in some interstitial cells of the cervix. No other cells including fibrocytes and lymphocytes were positive to MMP 9. No positive staining was found in control tissues. Conclusion There are strong expressions of MMP 9 in pregnant cervix in term labor, derived mainly from infiltrated PMN. MMP 9 may be an important regulator in the process of cervical ripening.