Three-Dimensional Culture of Hybridoma Cells Secreting Anti-Human Chorionic Gonadotropin by a New Rolling Culture System

Cell growth rate and production of monoclonal antibody (MAb) ofhybridoma cells producing anti-human chorionic gonadotropin (hCG)MAb have been used as investigation criteria in double-mouthedrolling bottle (DMRB). Compared with T-flask cell culture, bothof the cell number and MAb production increased by approximately42.5% when the medium was supplemented with 5% fetal calf serum(FCS) and DMRB rotated at 2 turns per minute. Yield of MAb wasexperimentally related to the number of viable cells.Interestingly, MAb yield was four times as high as that culturedin T-flask in the first 24hours, and about 75% yield oftotal MAb was secreted by 48hours during the culture. Itappears that the promoted cell growth and MAb yield are resultedfrom the three-dimensional growth of hybridoma cells under asuitably revolving condition.