| 大鼠胚胎脊髓移植后增加损伤脊髓生长抑素mRNA的表达 |
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| 引用本文： | 张强,邹德威,王拥军. 大鼠胚胎脊髓移植后增加损伤脊髓生长抑素mRNA的表达[J]. 中国矫形外科杂志, 2005, 13(20): 1568-1569 |
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| 作者姓名： | 张强 邹德威 王拥军 |
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| 作者单位： | 1. 解放军第306医院骨科,北京,100101
2. 上海中医药大学脊柱病研究所 |
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| 摘 要： | [目的] 研究大鼠胚胎脊髓移植后是否能够影响生长抑素（SOM）mRNA的表达。[方法]将动物分为单纯半切洞损伤组（A组）、胚胎脊髓移植组（B组）。术后1、3、7、14、28d，采用原位杂交方法观察SOM mRNA的表达，采用计算机图像分析技术，进行定量分析。[结果] 胚胎脊髓移植组SOM mRNA蛋白表达明显多于单损伤组。[结论] 作者的研究表明胚胎脊髓移植后可使损伤脊髓SOM mRNA表达增多。
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| 关 键 词： | 脊髓损伤 脊髓内移植 胚胎脊髓 生长抑素 原位杂交 生长抑素mRNA 胚胎脊髓移植 损伤脊髓 移植后 大鼠 |
| 收稿时间： | 2005-04-09 |
| 修稿时间： | 2005-06-16 |
Fetal spinal cord transplantation increaseing expression of somatotitin mRNA after spinal cord injury of rats |
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| ZHANG Qiang,ZOU De-wei,WANG Yong-jun. Fetal spinal cord transplantation increaseing expression of somatotitin mRNA after spinal cord injury of rats[J]. The Orthopedic Journal of China, 2005, 13(20): 1568-1569 |
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| Authors: | ZHANG Qiang ZOU De-wei WANG Yong-jun |
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| Affiliation: | The Orthopedic Department,The 306^th Hospital of PLA, Beijing 100101 |
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| Abstract: | [Objective] To investigate the effect of the fetal spinal cord transplantation on expression of somatostatin(SOM) mRNA after hemisection of spinal cord injury in the rat.[Method] Experimental rats were divided into two grops: hemisection injury group(group A), hemisection injury and fetal spinal cord transplantation group(group B).[Result]Rats were killed at 1, 3, 7, 14, 28 days postoperatively. The spinal cord lesion and neighbor areas were examied by an in situ hybridization.The positive cells were quantitated by a computer image analysis system. Group A the number of cells expressing protein SOM mRNA were higher than that of group B.[Conclusion]The fetal spinal cord transplant was sufficient to increase the number of SOM mRNA positive cells. |
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| Keywords: | Spinal cord injury Intraspinal graft Fetal spinal cord Somatostatin In situhybridization |
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