BIM重组腺病毒转染对EGFR突变NSCLC细胞凋亡及BIM表达水平的影响

目的探讨BIM重组腺病毒转染对非小细胞肺癌（NSCLC）细胞株的凋亡诱导作用及机制。方法将NSCLC细胞株A549、H1650、H1975分别培养至对数生长期后随机分为三组,其中转染组以BIM重组腺病毒转染、阴性对照组（NC组）以不带BIM的空载体转染、空白对照组（BC组）不转染,其后继续培养48 h。采用Annexin PI单染法检测细胞凋亡率,Western blot法检测BIM蛋白表达。结果与NC组和BC组比较,转染组细胞凋亡率及BIM蛋白表达均显著增高,其中H1650、H1975细胞株均显著高于A549细胞株（P均〈0.01）。结论 BIM重组腺病毒转染对EGFR突变NSCLC细胞凋亡具有诱导作用,可能机制为上调细胞内BIM蛋白表达;此为NSCLC的基因治疗提供了依据。

Effect of recombinant BIM on apoptosis and BIM expression transfection in NSCLC with EGFR mutation

Objective To analyze the effect and the mechanism of the apoptosis in non-small cell lung cancer cell（NSCLC） transfected by recombinant BIM.Methods A549,H1650,H1975 in logarithmic growth phase were divided into three groups randomly,transfect group was transfected by recombinant BIM,negative control group（NC group） was transfected without recombinant BIM,black control group（BC group） was not transfected.After 48 h,apoptosis of cell strains transfected by recombinant BIM was measured by Annexin PI;expression of BIM transfected by recombinant BIM was detected by western blot assay.Results In contrast with NC and BC groups,the apoptosis rate and the expression level of transfect group increased observably,and which were higher in the H1650 and H1975 mutant cell lines than those in the A549 cells（P〈0.01）.Conclusion Recombinant BIM transfection can induce the apoptosis in NSCLC cells with EGFR mutations,which possibly caused by increasing the expression of BIM;this provides basis for the gene therapy of NSCLC.