DNA甲基化定量检测的新方法:甲基化敏感性高分辨率熔解分析

DNA甲基化被认为是新一代的肿瘤标志,其对肿瘤风险评估、早期诊断以及肿瘤预后都有极其重要的诊断价值,MS-HRM方法 是一种新的定量检测甲基化的方法 ,它是基于亚硫酸盐对DNA进行修饰后具有不同的碱基构成,导致甲基化和非甲基化模板扩增后的产物热稳定性不同而进行检测.本文阐述了该方法 在肿瘤和遗传性疾病检测中的应用及优点,并对其在临床分子检测中的应用前景进行了展望.

A new approach for quantitative assessment of DNA methylation:methylation-sensitive high resolution melting

Methylated DNA is considered as a new generation of tumor biomarker.It shows great value for evaluation of cancer risk,early detection and predicting prognosis.MS-HRM is a novel approach for quantitative assessment of methylation.It is based on the different base compositions after PCR amplification of bisulfite-modified DNA template,which give rise to different thermal properties of the PCR products originating from methylated or unmethylated target template.This article describs the advantages of this method and its role in the detection of cancer and inherit disease.The prospective application in the molecular diagnosis is also suggested.