三维连通多孔钛表面成骨能力的实验研究

目的：通过体外成骨细胞培养实验,评估单纯连通多孔钛材料和复合类骨磷灰石的连通多孔钛材料表面的成骨能力。方法：先将纯钛粉应用热等静压法制备出单纯连通多孔钛并分成2组（每组50件）,将其中一组按1.5倍模拟体液浸泡法制备出复合连通多孔钛。将两组多孔钛制成标准试件（5mm×5mm×4mm）并均置于24孔板内。取新生SD鼠颅骨的成骨细胞进行原代培养及传代,然后将传代后的成骨细胞接种于24孔板内的两组多孔钛试件中并继续培养。在培养期间进行细胞增殖性测定（MTT法,在培养第1、3、5、7、9天测定）以及碱性磷酸酶活性检测（在培养第7、14、21天测定）。培养7d后对2组多孔钛内的成骨细胞进行扫描电镜观察。结果：2组的MTT吸光值和ALP值均随着时间的延长而增加,但复合多孔钛组在MTT吸光值的增加幅度和速度以及ALP活性均优于单纯多孔钛组（P〈0.05）。培养7d后扫描电镜显示复合连通多孔钛试件内成骨细胞生长活跃,黏附牢靠。结论：三维连通多孔钛表面具备成骨活性,在多孔钛内壁复合类骨磷灰石后,这种成骨活性明显增强,更有利于成骨细胞在材料表面的黏附和增殖。

Research on the osteogenic ability of 3-Ds interconnected porous titanium

Objective: To evaluate the osteogenic ability of the three-dimensional interconnected porous titanium(TDIPT) coated with bonelike apatite,by the test of the culture of osteoblast in vitro. Methods: The pure TDIPT was prepared by the high temperature insostatic pressing(HIP) and was divided into two groups. One group of TDIPT was dipped into the 1.5 simulated body fluid(1.5SBF) and developed the TDIPT coated with bonelike apatite(The test group). Another group was control group (pure TDIPT). The porous titanium of the two groups were all made into standard parts (5 mm×5 mm×4 mm) and placed into the 24-hole plates. The osteoblasts were extracted from SD rat. After the primary culture and subculture of the osteoblast in vitro,the osteoblasts were inoculated into the samples in the 24-pole plate and cultured continually. MTT Cell Proliferation Assay was done on the 1st,3rd,5th,7th and 9th day respectively after inoculating. The ALP activity was tested on the 7th,14th and 21st day, respectively. The collected data were analyzed by use of the student t test. Results: The MTT value and the ALP activity increased with the increasing of the culture time in the two group,but those of the test group was more significant than those of the control group(P<0.05). Moreover,the osteoblast tachyauxesis and stable adhesion were observed in test group by SEM at the 7th day after culturing. Conclusion: The bioactivity of the TDIPT improves significantly after coating with the bonelike apatite onto the surface of the TDIPT.