高效液相色谱同时测定食品中5种添加剂

[目的]建立高效液相色谱(HPLC)同时测定食品中安赛蜜、糖精钠、苯甲酸、山梨酸和阿斯巴甜的方法。[方法]样品经去蛋白、脱色、超声提取、过滤膜等预处理,采用HPLC二极管分段多波长同时测定。[结果]方法线性良好,相关系数为0.9995~0.9999,回收率为90.70%~98.20%,相对标准偏差为0.41%~2.40%。[结论]方法准确可靠、简便快速、易于掌握、便于推广。

Simultaneous determination of 5 additives in foods by HPLC

[Objective] To develop a method for detection of acesulfame - K, saccharin sodium, benzoic acid, sorbic acid and aspartame in foods by high performance liquid chromatography （ HPLC ）. [ Methods ] The food samples were deproteinized, decolored, ultrasonic extracted, filtered and detected simultaneously using DAD un- der optimal chromatography conditions. [ Results ] A good linear relationship was obtained and the correlation coefficient was 0. 9995 - 0. 9999. The rate of recovery was 90.70% - 98.20% and relative standard deviation （ RSD ） was 0.41% -2.40%. [ Conclusion ] This method is simple,rapid,reliable and accurate. It is suitable for popularized application.