Both phasic sensory stimulation and tonic pharmacological activation increase Fos-like immunoreactivity in the rat locus coeruleus. |
| |
Authors: | S J Grant K Bittman R H Benno |
| |
Affiliation: | Department of Psychology, University of Delaware, Newark 19716. |
| |
Abstract: | Neuronal activation increases levels of Fos protein, the product of the early immediate gene c-fos. Since most studies used stimuli that evoke sustained elevations in activity; the present study examined Fos-like immunoreactivity (Fos-LI) in the nucleus locus coeruleus (LC). Halothane-anesthetized rats were given either footshock to elicit phasic activation of LC neurons or yohimbine (10 mg/kg, i.p.) to induce a tonic increase in firing. Both treatments markedly increased Fos-LI in a subpopulation of LC neurons. These results demonstrate that c-fos induction does not require high tonic levels of neuronal activity and that Fos-LI may underestimate the proportion of LC neurons neurophysiologically activated by a given stimulus and suggest that factors beyond neuronal activity per se contributes to c-fos expression. |
| |
Keywords: | Rats Norepinephrine c-fos Immediate early gene Yohimbine α2-adrenergic receptor |
|