| 高效液相色谱法测定复方鱼腥草口服液中黄芩苷和绿原酸含量 |
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| 引用本文： | 陈波.高效液相色谱法测定复方鱼腥草口服液中黄芩苷和绿原酸含量[J].中国药业,2009,18(20):30-31. |
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| 作者姓名： | 陈波 |
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| 作者单位： | 江苏省镇江药品检验所,江苏,镇江,212003 |
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| 摘 要： | 目的建立复方鱼腥草口服液中黄芩苷和绿原酸的含量测定方法。方法采用Shimadzu C18柱（250mm×4．6mm，5μm），黄芩苷测定以甲醇-水-磷酸（45：55：0．2）为流动相，检测波长为315nm；绿原酸测定以乙腈-0．4％磷酸溶液（13：87）为流动相，检测波长为327nm。结果黄芩苷进样量在0．2654—1．3270μg范围内与峰面积线性关系良好，r=0．99996（n=5），平均回收率为100．5％，RSD=1．08％（n=6）；绿原酸进样量在0．1072～0．5360μg范围内与峰面积线性关系良好，r=0．99998（n=5），平均回收率为97．26％，RSD=1．41％（n=6）。结论该方法快速准确、结果可靠。
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| 关 键 词： | 复方鱼腥草口服液 黄芩苷 绿原酸 高效液相色谱法 含量测定 |
Determination of Baicalin and Chlorogenic Acid in Compound Yuxincao Oral Liquid by HPLC |
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| Chen Bo.Determination of Baicalin and Chlorogenic Acid in Compound Yuxincao Oral Liquid by HPLC[J].China Pharmaceuticals,2009,18(20):30-31. |
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| Authors: | Chen Bo |
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| Institution: | Chen Bo (Zhenjiang Institute for Drug Control, Zhenjiang, Jiangsu, Chino 212003) |
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| Abstract: | Objective To develop a method of determining the content of baicalin and chlorogenic acid in Compound Yuxincao Oral Liquid by HPLC. Methods The Shimadzu C18 column（250 mm×4.6mm, 5 μm） was used. Baicalin： the mobile phase consisted of methanol-water-phosphoric acid （45：55：0.2）. The detection wavelength was at 315 nm. Chlorogenic acid： the mobile phase consisted of acetonitrile0.4% phosphoric acid solution（13 ： 87）.The detection wavelength was at 327 nm. Results There was a good linearity for baicalin in the range of 0. 265 4 - 1. 327 0 μg （ r = 0. 999 96, n = 5）. The average recovery rate was 100. 5% , RSD = 1.08% （ n = 6）. There was a good linearity for chlorogenic acid in the range of 0. 107 2-0.5360 p,g, r=0.999 98（n=5）. The average recovery rate was 97.26%, RSD= 1.41% （n =6）. Conclusion The method is rapid and accurate with reliable results. It also improves the quality standard. |
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| Keywords: | Compound Yuxincao Oral Liquid baicalin chlorogenic acid HPLC content determination |
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