Innate immune system plays a critical role in determining the progression and severity of acetaminophen hepatotoxicity |
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Authors: | Liu Zhang-Xu Govindarajan Sugantha Kaplowitz Neil |
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Affiliation: | Research Center for Liver Disease, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA. zxliu@usc.edu |
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Abstract: | BACKGROUND & AIMS: Inflammatory mediators released by nonparenchymal inflammatory cells in the liver have been implicated in the progression of acetaminophen (APAP) hepatotoxicity. Among hepatic nonparenchymal inflammatory cells, we examined the role of the abundant natural killer (NK) cells and NK cells with T-cell receptors (NKT cells) in APAP-induced liver injury. METHODS: C57BL/6 mice were administered a toxic dose of APAP intraperitoneally to cause liver injury with or without depletion of NK and NKT cells by anti-NK1.1 monoclonal antibody (MAb). Serum alanine transaminase (ALT) levels, liver histology, hepatic leukocyte accumulation, and cytokine/chemokine expression were assessed. RESULTS: Compared with APAP-treated control mice, depletion of both NK and NKT cells by anti-NK1.1 significantly protected mice from APAP-induced liver injury, as evidenced by decreased serum ALT level, improved survival of mice, decreased hepatic necrosis, inhibition of messenger RNA (mRNA) expression for interferon-gamma (IFN-gamma), Fas ligand (FasL), and chemokines including KC (Keratinocyte-derived chemokine); MIP-1 alpha (macrophage inflammatory protein-1 alpha); MCP-1 (monocyte chemoattractant protein-1); IP-10 (interferon-inducible protein); Mig (monokine induced by IFN-gamma) and decreased neutrophil accumulation in the liver. Hepatic NK and NKT cells were identified as the major source of IFN-gamma by intracellular cytokine staining. APAP induced much less liver injury in Fas-deficient (lpr) and FasL-deficient (gld) mice compared with that in wild-type mice. CONCLUSIONS: NK and NKT cells play a critical role in the progression of APAP-induced liver injury by secreting IFN-gamma, modulating chemokine production and accumulation of neutrophils, and up-regulating FasL expression in the liver, all of which may promote the inflammatory response of liver innate immune system, thus contributing to the severity and progression of liver injury downstream of the metabolism of APAP and depletion of reduced glutathione (GSH) in hepatocytes. |
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Keywords: | APAP, acetaminophen AsGM1, asialo-GM1 B6, C57BL/6 FasL, Fas ligand FITC, fluorescein isothiocyanate GKO, IFN-γ gene-deleted mice gld, FasL-deficient mice GSH, reduced glutathione IFN-γ, interferon-γ KC, Keratinocyte-derived chemokine lpr, Fas-deficient mice MAb, monoclonal antibody MCP-1, monocyte chemoattractant protein-1 MHC, major histocompatibility complex MIP, macrophage inflammatory protein NK, natural killer (cell) NKT, natural killer cells with T-cell receptors PCR, polymerase chain reaction PE, phycoerythrin TNF, tumor necrosis factor TRAIL, tumor necrosis factor-related apoptosis-inducing ligand |
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