| 辛伐他汀对THP-1巨噬细胞源性泡沫细胞胆固醇代谢和SR-A表达的影响 |
| |
| 引用本文： | 李晨光,罗俊生,冯旭,霍小川,关宁.辛伐他汀对THP-1巨噬细胞源性泡沫细胞胆固醇代谢和SR-A表达的影响[J].山东医药,2011,51(17):22-24. |
| |
| 作者姓名： | 李晨光 罗俊生 冯旭 霍小川 关宁 |
| |
| 作者单位： | 辽宁医学院附属第一医院,辽宁锦州,121001 |
| |
| 基金项目： | 国家自然科学基金，辽宁省自然科学基金 |
| |
| 摘 要： | 目的探讨不同浓度辛伐他汀对THP-1巨噬细胞源性泡沫细胞胆固醇代谢和SR-A表达的影响。方法以50 nmol/L佛波酯诱导THP-1细胞分化成巨噬细胞,氧化型低密度脂蛋白（ox-LDL）诱导巨噬细胞建立泡沫细胞模型,通过不同浓度辛伐他汀干预后,油红O染色观察细胞内脂质堆积情况,高效液相色谱检测细胞内游离胆固醇和胆固醇酯含量,W estern b lot检测SR-A蛋白的表达。结果与ox-LDL组比较,辛伐他汀处理组巨噬细胞的油红O染色阳性细胞数和细胞内胆固醇酯含量均显著减少（P均〈0.01）,同时SR-A蛋白表达明显下降（P〈0.01）,呈剂量依赖性。结论辛伐他汀可抑制ox-LDL诱导的THP-1巨噬细胞泡沫化,可能与辛伐他汀抑制SR-A的表达,从而减弱巨噬细胞对ox-LDL的摄取有关。
|
| 关 键 词： | 辛伐他汀 脂蛋白类 LDL 单核细胞 泡沫细胞 清道夫受体 |
Effect of Simvastain on cholesterol metabolism and SR-A in THP-1 foam cell |
| |
| LI Chen-guang,LUO Jun-sheng,FENG Xu,HUO Xiao-chuan,GUAN Ning.Effect of Simvastain on cholesterol metabolism and SR-A in THP-1 foam cell[J].Shandong Medical Journal,2011,51(17):22-24. |
| |
| Authors: | LI Chen-guang LUO Jun-sheng FENG Xu HUO Xiao-chuan GUAN Ning |
| |
| Institution: | LI Chen-guang,LUO Jun-sheng,FENG Xu,HUO Xiao-chuan,GUAN Ning(First Affiliated Hospital of Liaoning Medical College,Jinzhou 121001,P.R.China) |
| |
| Abstract: | Objective To study the effect of Simvastatin on the expression of scavenger receptor class A(SR-A) and the cholesterol metabolism in THP-1 macrophage-derived foam cells.Methods The THP-1 cells were stimulated by phorbol 12-myristate 13-acetate(50 nmol/L) for 48 hours and transformed to macrophages.Macrophages were co-incubated with 100 mg/L oxidized low density lipoprotein(ox-LDL) and simvastatin.The cellular lipid accumulation was examined by oil red O staining.High performance liquid chromatography was us... |
| |
| Keywords: | Simvastatin lipoproteins LDL monocytes foam cells scavenger receptor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
