慢性再生障碍性贫血患者的细胞因子水平与中医证型的相关性研究

目的研究慢性再生障碍性贫血(AA)患者的细胞因子水平与中医证型的相关性。方法采用双抗体夹心ELISA法检测34例慢性AA患者的外周血γ干扰素(IFN-γ)、肿瘤坏死因子(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-3(IL-3)、白细胞介素-10(IL-10)和转化生长因子β(TGF-β)水平。结果 AA患者肾阴虚型和肾阳虚型与细胞分子的关系分析,IL-2在肾阳虚组中明显升高,在肾阴虚证中明显降低(P=0.008)。中医证型与观察指标分层讨论中显示,肾阳虚型和肾阴虚型在细胞因子的变化上有明显不同,这可能进一步说明包括IFN-γ、TNF-α、IL-2、IL-3、IL-10和TGF-β在内的多种细因子与中医辨证的内在关系,虽然不是所有的一一对等,但具有明显的统计学差异。结论中医证型与IFN-γ、TNF-α、IL-2、IL-3、IL-10和TGF-β存在明显相关性,IFN-γ、TNF-α、IL-2、IL-3、IL-10和TGF-β可能作为中医辨证的客观参考依据。

Correlative Study between Cytokines and TCM Patterns/Syndromes in the Patients of Chronic Aplastic Anemia

Objective To study the correlation between cytokine level and TCM patterns/ syndromes in the patients of chronic aplastic anemia（CAA）. Methods The double - antibody sandwich ELISA method was adopted to determine the levels of IFN - γ,TNF - α,IL - 2,IL - 3,IL - 10 and TGF - β in the peripher-al blood of 34 CAA patients. Results In analysis on the correlation in CAA patients of kidney yin deficiency pattern and kidney yang deficiency pattern with cytokine,the results showed that IL - 2 was increased obvi-ously in kidney yang deficiency group and was reduced apparently in kidney yin deficiency group（ P =0. 008）. The hierarchic discussion between TCM patterns/ syndromes and observation indexes showed that the changes in cytokines were obviously different between kidney yang deficiency pattern and kidney yin deficien-cy pattern. It further explained possibly the relationship of many cytokines including IFN - γ,TNF - α,IL -2,IL - 3,IL - 10 and TGF - β with TCM pattern/ syndrome differentiation,but indicating the obviously signif-icant differences. Conclusion TCM patterns/ syndromes are correlated apparently with IFN - γ,TNF - α,IL- 2,IL - 3,IL - 10 and TGF - β. IFN - γ,TNF - α,IL - 2,IL - 3,IL - 10 and TGF - β are possibly taken as the objective references to the pattern/ syndrome differentiation in TCM.