电针对慢性应激模型大鼠下丘脑及胃肠道cAMP、PKA和PKC的调控作用

目的从受体后信号转导通路上重要调节因子环磷酸腺苷(cAMP)、蛋白激酶A(PKA)、蛋白激酶C(PKC)的角度,探讨慢性应激状态下大鼠下丘脑、胃肠道受体后信号转导的变化及电针百会、印堂、天枢穴改善抑郁症消化功能障碍的作用机理。方法将40只雄性SD大鼠随机分为正常组、模型组、对照组、电针组,每组10只。采用放射性同位素等方法,观察慢性应激模型大鼠下丘脑、胃窦及空肠组织的cAMP含量、PKA及PKC活性的变化及电针印堂、百会和天枢穴的干预作用。结果模型组大鼠下丘脑、胃窦及空肠组织cAMP含量及PKA活性明显低于正常组,PKC活性明显高于正常组,差异有统计学意义(P0.01);电针组及对照组cAMP含量及PKA活性明显均较模型组升高,PKC活性较模型组明显降低,两组与模型组比较,差异有统计学意义(P0.01);电针组与对照组比较无明显差别。结论抑郁模型大鼠下丘脑、胃窦及空肠组织受体后cAMP、PKA信号通路下调,而PKC信号通路上调,电针改善抑郁症伴发的消化功能障碍可能与调节受体后cAMP、PKA及PKC信号通路有关。

Regulatory Effect of Electroacupuncture on cAMP,PKA and PKC in Hy-pothalamus and Gastrointestinal Tract in the Model Rats of Chronic Stress

Objective To explore the changes in the signal transduction pathway of hypothalamic and gastrointestinal receptors as well as the effect mechanism on digestive disturbance in depression treated with electroacupuncture（EA）at Baihui（GV20）,Yintang（EX - HN3）and Tianshu（ST25）in terms of the im-portant regulatory factors such as cAMP,PKA and PKC. Methods Forty male SD rats were randomized into a normal group,a model group,a control group and an EA group,10 rats in each one. The radioisotope method was used to observe cAMP level,activity of PKA and PKC in hypothalamus,antrum and jejunum,as well as the intervention of EA at Yintang（EX - HN3）,Baihui（GV20）and Tianshu（ST25）. Results In the model group,cAMP level and PKA activity in hypothalamus,antrum and jejunum were lower apparently than those in the normal group in the rats,PKC activity was higher apparently than that in the normal group,indicating the significant difference（P ﹤ 0. 01）. The cAMP level and PKA activity in EA group and the control group were all increased apparently than those in the model group,but PKC activity was reduced apparently than that in the model group. The differences were significant in comparison of these two groups with the model group（P ﹤ 0. 01）. The difference was not significant between EA group and the control group. Conclusion The posterior signal transduction pathway,cAMP and PKA were down - regulated and PKC was up - regula-ted in hypothalamus,antrum and jejunum in the model rats of depression. EA treats digestive disturbance in depression,which is likely related to its regulatory effect on cAMP,PKA and PKC signal transduction.