针灸对IBS内脏痛大鼠结肠肌间神经丛神经元P2X_2受体表达的调节

目的观察针灸对肠易激综合征(IBS)内脏痛大鼠结肠肌间神经丛神经元嘌呤受体亚型P2X2表达的调节。方法应用直结肠球囊扩张刺激法制备慢性内脏高敏感IBS大鼠模型,随机分为正常组、模型组、电针组,电针组采用电针"天枢""上巨虚"穴进行治疗。治疗结束后,进行腹部撤回反射评分(AWR),应用免疫荧光法检测肠结肠肌间神经丛P2X2表达变化。结果模型大鼠AWR评分升高,电针治疗后降低;模型组大鼠结肠肌间神经丛神经元中P2X2表达与正常组大鼠相比异常增加,电针组显著降低。结论电针(天枢、上巨虚)能抑制结肠肌间神经丛神经元P2X2受体异常表达,改善IBS大鼠的内脏痛觉敏感状态。

Expression of P2X2 Receptors in Myenteric Plexus Neurons Regulated with Acupuncture in the Rats with IBS Visceral Pain

Objective To observe the regulation of acupuncture on the expression of P2X2 receptors in myenteric plexus neurons in the rats with IBS visceral pain. Methods The rat model of chronic visceral pain in high sensitive IBS（irritable bowel syndrome）was prepared by rectal balloon dilation stimulation meth-od. The normal group,model group and electroacupuncture（EA）group were randomized. In EA group,EA was applied to Tianshu（ST25）and Shangjuxu（ST37）. At the end of treatment,abdominal withdrawal reflection score（AWRs）was given. The immunofluorescence was applied to determine the changes in the expression of P2X2 receptors in myenteric plexus neurons in the rats with IBS visceral pain. Results AWRs was increased in model rats,was decreased after EA. In the model group,the expression of P2X2 receptors in myenteric plexus neurons was abnormally increased as compared with the normal group. It was significantly decreased in EA group. Conclusion EA（ST25,ST37）inhibits the abnormal expression of P2X2 receptors in myenteric plexus neurons and improves the sensitive state of visceral pain in IBS rats.