反相高效液相色谱法测定心脑康胶囊中丹参酮ⅡA含量

目的用反相高效液相色谱（RP-HPLC）法测定心脑康胶囊的丹参酮ⅡA含量。方法色谱柱Hypersil BDS C18柱（200mm×4．6mm，5μm），以甲醇-水（80：20）为流动相，检测波长为269nm，流动相流速为1．0mL／min，柱温为25℃。结果丹参酮ⅡA进样量线性范围为0．02-0．10μg（r=0．9990），平均加样回收率为98、55％，RSD=1．26％（n=6）。结论RP-HPLC法具有简便、灵敏、准确、重现性好等优点，适合于心脑康胶囊中丹参酮ⅡA的含量测定。

Determination of Tanshinone ⅡA in Xinnaokang Capsule by RP-HPLC

Objectlve To establish a RP-HPLC method for the quantitative determination of Tanshinone ⅡA in Xinnaokang Capsule. Methods Reversed phase high performance liquid chromatography（RP-HPLC） was used with Hypersil BDS C18 （200 mm×4.6 mm,5 μm） column as the chromato graphic column. The mobile phase was mathanol water （80：20）. The detecting wavelength was 269 nm, the flow rate was 1.0 mL/min, the column temperature was 25℃. The external standard method was employed. Results The calibration curves were linear within the range of 0.02-0.10μg （r=0.999 0） for tanshinone ⅡA. The average recovery rate was 98.55% with RSD=1. 26% （n =6）. Conclusion The method is simple,accurate,highly sensitive and reproducible It can be used for quantitative determination of the tanshinone ⅡA in Xinnaokang Capsule.