IL-1β和TNF-α基因多态性与慢性牙周炎的相关性分析

目的探讨细胞因子基因多态性与中、重度慢性牙周炎遗传易感性的关系。方法采用PCR-RFLP方法检测IL-1B 3953和TNF-Α-308基因型。采用多变量Logistic回归模型,确定IL-1B 3953和TNF-Α-308等位基因2阳性基因型对中、重度慢性牙周炎的影响。结果中、重度慢性牙周炎组IL-1B 3953(28.23%)和TNF-Α-308(29.03%)等位基因2阳性基因型出现的频率和对照组(分别为13.95%和11.05%)相比有显著性差异(P<0.01)。多变量Logistic回归模型分析结果显示,携带等位基因2阳性基因型的个体较携带阴性型个体发生中、重度慢性牙周炎的危险性相对较大,且统计学有显著意义(IL-1B 3953和TNF-Α-308分别为P<0.05,P<0.01)。结论IL-1B 3953和TNF-Α-308等位基因2阳性基因型可能与中、重度慢性牙周炎易感性有关。

Study on the Correlation of Cytokine Gene Polymorphism with Chronic Periodontitis

OBJECTIVE: To study the correlation of gene polymorphism IL-1B +3953 and TNF-A-308 and the genetic susceptibility to moderate-to-advanced adult periodontitis(AP). METHODS: PCR-RFLP technique was used to identify IL-1B +3953 and TNF-A-308 genotype. Multivariate logistic regression model was utilized to assess the relationship of IL-1B +3953 and TNF-A-308 positive genotype to disease status while adjusting for other potential confounders. RESULTS: The frequency of IL-1B +3953 (28.23%) and TNF-A-308 (29.03%) allele 2 positive genotype in patients with moderate-to-advanced AP showed a statistically significant increase compared to the control group frequency (13.95% IL-1B +3953 and 11.05% TNF-A-308), and the difference was of much significance (P < 0.01 in both group). Multivariate logistic regression analysis showed that IL-1B +3953 and TNF-A-308 allele 2 positive genotype may increase the odds of having moderate-to-advanced AP and the IL-1B +3953 (P < 0.05) and TNF-A-308 (P < 0.01) allele 2 positive genotype was of statistical significance. CONCLUSIONS: IL-1B +3953 and TNF-A-308 allele 2 positive genotype have closer correlation with moderate-to-advanced AP.